Sequali report

Table of contents

Links

Metadata

FilenameGM24385_1.fastq.gz
Filesize27.16 GiB
Sequali version1.0.1

Summary

Mean length 9,115.18
Length range (min-max) 3 497,187
Total reads 2,900,987
Q20 reads 0 0.00%
Total bases 26,443,022,791
Total GC bases 10,703,095,094 40.48%
Q20 bases 14,645,135,876 55.38%

Sequence length distribution

See this wikipedia lemma for an explanation about contiguity statistics.

PercentileRead length
118
528
1035
2554
501,039
756,442
9027,121
9553,491
99106,963
ContiguityRead length
N906,888
N5050,349
Sequence length distribution00100001000020000200003000030000400004000050000500006000060000700007000080000800009000090000100000100000110000110000120000120000130000130000140000140000150000150000027591001331772373164225647531006134417972401320842885730765810234136781828024431326524363958323779481041771392331860842487013323894442380022.2682051292114547.624090954578801-513927.329160840395804547.384518802388416-10279832.39011655158022542.80162417883611-151306637.45107226276462525.10430864866931116-203264242.51202797394903491.364276625059631621-267241147.57298368513343422.82078798862042127-318804852.63393939631785395.86978263751782732-3610806157.694895107502255361.37656334407173237-4111851362.75585081868665343.362116331874743742-4612037867.81680652987107340.147712994929864247-5315575172.87776224105548279.1809091140994754-589331277.93871795223988386.797064586216155459-649014882.9996736634243392.25034724471135965-706763988.06062937460871431.04552403360746571-786221693.12158508579311440.39228505036447179-853590198.18254079697752485.74725760355667986-9018196103.24349650816193516.26254504624198691-9922550108.30445221934635508.7582489984897691100-1048517113.36540793053076532.9446951257535100105-1096697118.42636364171517536.0815391328525105110-1145341123.48731935289956538.4186602722076110115-1204958128.54827506408398539.078776346229115121-1264007133.60923077526837540.7178635169713121127-1323409138.67018648645276541.7485408335896127133-1393478143.73114219763718541.6296165278259133140-1452649148.79209790882157543.0584317376529140146-1533136153.85305362000602542.2190674346543146154-1602489158.9140093311904543.3341982437714154161-1682653163.9749650423748543.0515375749999161169-1762435169.03592075355922543.4272694395866169177-1852754174.09687646474364542.8774599680125177186-1942620179.15783217592804543.108414416887186195-2042998184.21878788711246542.4569160461817195205-2143017189.27974359829685542.42416877358205215-2253236194.34069930948127542.0467133683303215226-2363367199.40165502066566541.8209295414457226237-2473307204.4626107318501541.9243419812401237248-2604015209.52356644303447540.7040751916654248261-2734152214.5845221542189540.4679501208013261274-2864072219.6454778654033540.6058333738606274287-3004675224.7064335765877539.5665383539263287301-3155180229.76738928777212538.6961503189893301316-3315764234.82834499895654537.6896025716566316332-3476012239.88930071014093537.2621644871728332348-3646927244.95025642132535535.685124780307348365-3827498250.01121213250983534.7009830615964365383-4018321255.07216784369416533.2825090957488383402-4218836260.1331235548786532.3948856541796402422-4428813265.19407926606306532.4345270894341422443-4648860270.25503497724736532.3535206782618443465-4878814275.3159906884318532.4328035487708465488-5118862280.37694639961626532.3500735969353488512-5369318285.4379021108007531.5641390544973512537-56310374290.498857821985529.7440801141147537564-59110716295.55981353316946529.1546292072861564592-62011041300.6207692443539528.5944784917327592621-65111166305.68172495553824528.3790359088276621652-68310830310.74268066672266528.9581455716766652684-71711600315.8036363779071527.6310192609809684718-75212083320.86459208909145526.7985491206355718753-78912712325.9255478002759525.7144420434568753790-82812755330.98650351146034525.6403297949373790829-87012743336.0474592226447525.6610122828962829871-91313145341.1084149338291524.9681489362733871914-95813948346.16937064501354523.5841457836906914959-100514567351.23032635619796522.51727411314439591006-105514547356.2912820673823522.551744926409110061056-110715187361.35223777856675521.448678901934710561108-116216038366.41319348975117519.981945797516511081163-122016270371.47414920093564519.582084363644511631221-128016360376.53510491211995519.426965703952912211281-134316625381.59606062330437518.970227428193912811344-141017554386.65701633448884517.369058152042813441411-148017540391.7179720456732517.393187721328214111481-155317842396.77892775685757516.872678441029414811554-163017986401.83988346804205516.624488585522615541631-171118301406.9008391792264516.081573276601616311712-179618401411.96179489041083515.909219210277617121797-188518868417.0227506015953515.104325720543917971886-197818914422.08370631277967515.025042850034818861979-207619517427.14466202396403513.985747830100319792077-217919343432.2056177351485514.285643905504320772180-228719738437.2665734463329513.604845343524121802288-240019703442.32752915751723513.665169266737522882401-251919926447.3884848687017513.280819698834624012520-264319735452.44944057988613513.610015965513825202644-277419919457.5103962910705513.292884483477326442775-291220054462.5713520022549513.060206493939927752913-305620240467.63230771343933512.73962793057729133057-320720109472.69326342462375512.965411757461530573208-336619897477.75421913580817513.330802378068732083367-353320328482.81517484699253512.587956352211733673534-370820450487.87613055817695512.377684391296335343709-389220412492.9370862693614512.443178936499437093893-408420365497.9980419805458512.524185347671838934085-428720551503.0589976917301512.203606784308940854288-449920641508.1199534029146512.048488124617342884500-472220739513.180909114099511.8795811396196345004723-495620832518.2418648252833511.719291857938247234957-520120866523.3028205364677511.66069147538849575202-545920894528.3637762476521511.612432336817252025460-572920698533.4247319588367511.9502463068124654605730-601320521538.485687670021512.255313004206257306014-631120463543.5466433812054512.355278362674260146312-662320279548.6075990923898512.672409844710563126624-695119995553.6685548035742513.161895393071166246952-729519713558.7295105147585513.64793386010569527296-765719233563.7904662259431514.475233378460972967658-803618700568.8514219371274515.393880551968476588037-843417518573.9123776483118517.431105615919580378435-885216994578.9733333594962518.334240923457984358853-929016291584.0342890706806519.545890009716588539291-975015954589.0952447818651520.126723213228792919751-1023315463594.1562004930496520.9729816788802975110234-1074015407599.217156204234521.06949995602171023410741-1127215434604.2781119154182521.02296435811421074111273-1183015282609.3390676266026521.28494253892681127311831-1241614927614.400023337787521.89679947437741183112417-1303114768619.4609790489715522.17084243983281241713032-1367714287624.521934760156522.99986549885181303213678-1435414101629.5828904713404523.32044406221461367814355-1506513784634.6438461825248523.86680645246211435515066-1581113416639.7048018937091524.50106941653481506615812-1659513051644.7657576048935525.1301617586181581216596-1741612847649.8267133160779525.48176405391921659617417-1827912511654.8876690272624526.06087371676811741718280-1918412357659.9486247384468526.32629897890731828019185-2013512237665.0095804496312526.53312385849631918520136-2113211855670.0705361608155527.19151639185442013621133-2217911527675.131491872527.75683772939752113322180-2327711290680.1924475831844528.16531686658572218023278-2443011188685.2534032943688528.34111801423632327824431-2564010962690.3143590055532528.73063820412882443125641-2691010775695.3753147167376529.05294030815492564126911-2824310777700.4362704279221529.04949322682842691128244-2964210646705.4972261391064529.2752770537132824429643-3111010550710.5581818502908529.44073695738422964331111-3265110322715.6191375614752529.83370422860323111132652-3426810338720.6800932726597529.80612757799133265234269-3596510227725.7410489838442529.9974405916113426935966-3774710298730.8020046950285529.8750692045213596637748-3961610301735.8629604062129529.86989858253133774839617-4157810298740.9239161173972529.8750692045213961741579-4363810282745.9848718285816529.90264585513274157943639-4579910231751.0458275397662529.99054642895824363945800-4806810238756.1067832509506529.97848164431554580048069-504489961761.167738962135530.45590240803324806950449-5294710209766.2286946733194530.02846432354945044952948-5556910106771.2896503845038530.20598901186335294855570-583229966776.350606095688530.4472847047175557058323-612109808781.4115618068726530.71960412950925832361211-642429443786.472517518057531.34869647159216121164243-674249303791.5334732292414531.58999216444596424367425-707648930796.5944289404258532.23287283183496742570765-742698677801.6553846516102532.66892861963497076574270-779478384806.7163403627945533.17392603396467427077948-818087862811.7772960739791534.07361426017667794881809-858607598816.8382517851634534.52862899527218180985861-901127061821.8992074963478535.45417033143278586190113-945766605826.9601632075322536.24010487387079011394577-992605997832.0211189187166537.28801759712139457799261-1041765571837.0820746299011538.02224591966299261104177-1093364950842.1430303410855539.0925646715349104177109337-1147524556847.2039860522699539.771639692852109337114753-1204353827852.2649417634543541.0281008363547114753120436-1264003436857.3258974746387541.702005235682120436126401-1326612941862.386853185823542.5551578639864126401132662-1392322513867.4478088970075543.2928332678537132662139233-1461282113872.508764608192543.9822495331501139233146129-1533661720877.5697203193763544.6596010138039146129153367-1609621373882.6306760305607545.2576696239487153367160963-1689341081887.6916317417451545.760943497615160963168935-177302912892.7525874529296546.0522218697029168935177303-186083689897.813543164114546.4365714376056177303186084-195300479902.8744988752984546.7985149768863186084195301-204973367907.9354545864827546.9915515311693195301204974-215126259912.9964102976671547.1776939227993204974215127-225781216918.0573660088515547.2518061713188215127225782-236964147923.118321720036547.3707304770824225782236965-24870093928.1792774312204547.4638016728974236965248701-26101956933.2402331424049547.5275726774373248701261020-27394743938.3011888535893547.5499787060594261020273948-28751524943.3621445647736547.5827259786611273948287516-30175614948.423100275958547.5999613852935287516301757-31670212953.4840559871424547.6034084666198301757316703-3323885958.5450116983268547.6154732512625316703332389-3488514963.6059674095113547.6171967919258332389348852-3661303968.6669231206957547.6189203325891348852366131-3842643973.7278788318802547.6189203325891366131384265-4032972978.7888345430645547.6206438732523384265403298-4232720983.8497902542489547.6240909545788403298423273-4442370988.9107459654333547.6240909545788423273444238-4662401993.9717016766176547.6223674139155444238466241-4893332999.0326573878021547.6206438732523466241489334-51356911004.0936130989865547.6223674139155489334Sequence length distributionsequence lengthnumber of readsLength

Per position quality score distribution

Per base quality distribution000.10.10.20.20.30.30.40.40.50.50.60.60.70.70.80.80.90.911132651051401862483324435927901056141118862520336745006014803710741143551918525641342694580061211818091093371461291953012610203488524662411-50.337468219323.03672067723784457.0330483947367416-100.369198295828.296245946013602448.5153257009909611-150.292938105133.55577121478937468.98685395177961116-200.281281361638.81529648356513472.11602725276021621-260.299405451144.07482175234089467.25073898852512127-310.321779874349.33434702111665461.24447763949582732-360.297046139154.59387228989242467.88408023243423237-410.289675999859.85339755866818469.862543878715543742-460.284731671265.11292282744394471.18981517286524247-530.274882024970.37244809621971473.83388555365264754-580.262807195775.63197336499546477.07529113696465459-640.251655147180.89149863377122480.06898253359235965-700.240620769486.151023902547483.03108599781766571-780.230483759391.41054917132276485.75229724574897179-850.222123956496.6700744400985487.99642932731467986-900.2178140721101.92959970887429489.1533884017868691-990.2144149739107.18912497765004490.0658531174521591100-1040.2122872821112.4486502464258490.63701749384336100105-1090.2112444059117.70817551520157490.91697049707705105110-1140.2104163924122.96770078397732491.13924507601314110115-1200.2094893866128.22722605275308491.3880934598473115121-1260.2090991091133.48675132152886491.49286078833256121127-1320.2082273061138.7462765903046491.7268903525271127133-1390.2070772523144.00580185908035492.0356144635734133140-1450.2059295835149.26532712785612492.34369833738947140146-1530.2039978346154.5248523966319492.862263150639146154-1600.2017911631159.78437766540765493.4546290662673154161-1680.1994437798165.04390293418342494.0847680947991161169-1760.1972546713170.30342820295917494.67241934671716169177-1850.1953453863175.56295347173494495.184953907612177186-1940.1938651665180.8224787405107495.58230881255736186195-2040.1929529123186.08200400928646495.82719723092623195205-2140.192534718191.34152927806224495.9394586396195205215-2250.1920797838196.60105454683801496.06158263546246215226-2360.1922301309201.86057981561373496.02122295845334226237-2470.1924141344207.1201050843895495.97182849797366237248-2600.1924434201212.37963035316528495.96396694021325248261-2730.1921051609217.63915562194106496.05477031495127261274-2860.1920752529222.8986808907168496.06279891160557274287-3000.1914315532228.15820615949258496.23559571503233287301-3150.1906194898233.41773142826833496.4535885872332301316-3310.1895195813238.6772566970441496.74885151841124316332-3470.1882524153243.93678196581988497.0890135996612332348-3640.1867275511249.19630723459562497.4983530011226348365-3820.1859847308254.4558325033714497.69775803527216365383-4010.1854141886259.71535777214723497.8509162202829383402-4210.1852820337264.97488304092303497.88639228291424402422-4420.1850099868270.2344083096987497.95942141456607422443-4640.1846238336275.4939335784745498.06308162822006443465-4870.1839609025280.7534588472503498.2410409647032465488-5110.1828143419286.012984116026498.5488273363284488512-5360.1816141616291.2725093848018498.87100754568235512537-5630.180470391296.53203465357757499.1780449766152537564-5910.1795874458301.7915599223533499.41506559544996564592-6200.1788914862307.0510851911291499.6018911944419592621-6510.177966527312.31061045990486499.85019017384616621652-6830.1766366384317.57013572868055500.207189711761652684-7170.175533145322.8296609974564500.5034149900205684718-7520.1741617214328.08918626623216500.87156429815127718753-7890.1732849196333.34871153500785501.10693577817796753790-8280.1723181851338.6082368037837501.3664490605222790829-8700.171342873343.8677620725594501.628264939391829871-9130.1701491483349.1272873413352501.94871219176054871914-9580.1691911312354.386812610111502.20588535601553914959-10050.1681527926359.6463378788867502.48462027320669591006-10550.1671715528364.9058631476625502.7480274100527710061056-11070.1660310293370.16538841643825503.054193174097610561108-11620.1651004745375.424913685214503.303994263172511081163-12200.1639771423380.68443895398974503.6055451152867611631221-12800.1629443782385.94396422276554503.882783619050812211281-13430.1619039776391.20348949154135504.162072051358212811344-14100.1608224529396.46301476031704504.4523999973613413441411-14800.1598220096401.72254002909284504.72096216401614111481-15530.1588419296406.9820652978686504.9840579571925314811554-16300.1578454433412.24159056664433505.251557920434215541631-17110.1566809251417.50111583542014505.5641648947895416311712-17960.155690895422.7606411041959505.8299317263978317121797-18850.1546082481428.02016637297163506.120560908473417971886-19780.1536344222433.27969164174743506.381977814133218861979-20760.1525741714438.5392169105232506.666594914096519792077-21790.1516302351443.7987421792989506.919988185727220772180-22870.1505504484449.05826744807473507.209849568163321802288-24000.1496604895454.3177927168505507.4487529625017722882401-25190.1486607824459.57731798562617507.7171175130355324012520-26430.1477270916464.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base quality distributionpositionfraction0-34-78-1112-1516-1920-2324-2728-3132-3536-3940-43>=44
Per base quality distribution on read start000.10.10.20.20.30.30.40.40.50.50.60.60.70.70.80.80.90.911111213141516171819110.20988753149.057692307692308510.3198229801417120.377600451212.680769230769231463.5537203505088230.417698183416.303846153846155452.372621933368340.295574395219.926923076923078486.42637058231156450.386581411323.550000000000004461.049414162875560.350718479627.17307692307693471.04965473756425670.322690964530.79615384615385478.8650195140592780.451396131834.41923076923077442.9760786254002890.40114736338.04230769230769456.987754557762069100.320031857941.66538461538462479.6065011719310310110.329271751745.28846153846155477.029992315787911120.280854621848.91153846153846490.530922757033512130.273712938452.53461538461538492.522353711822313140.311149738356.15769230769231482.0832460466444314150.269638337559.78076923076923493.658540498128515160.261045815263.403846153846146496.0545323114976716170.298579696567.02692307692307485.588353869459217180.279655819870.64999999999999490.8652040994594518190.268156934974.27307692307691494.071623919048319200.299065002477.89615384615384485.4530281818512720210.290146483181.51923076923077487.939922971800321220.287322284985.1423076923077488.7274397918203622230.307739729388.76538461538462483.0341139473688423240.300081817292.38846153846154485.1694932909598524250.29789486596.01153846153846485.7793164807272325260.313536386699.63461538461539481.4177383554786626270.3115332719103.25769230769232481.976299190381127280.3276515049106.88076923076925477.4817919100800628290.3428430104110.50384615384617473.2456990129087429300.3222115666114.12692307692308478.9986977836285330310.3045193278117.75483.9321105055796631320.305058578121.37307692307692483.781742685753432330.2977481912124.99615384615386485.820215921193633340.2929647132128.6192307692308487.1540703559153434350.2964791109132.24230769230772486.1740940894111435360.2927743089135.8653846153846487.207163856964736370.2896716569139.48846153846154488.072326427820737380.2923127392143.11153846153846487.335870799512138390.2894768789146.73461538461538488.126639542007339400.2875070519150.35769230769233488.675918225670140410.2893612663153.98076923076925488.1588776773419341420.2873380173157.60384615384618488.723052878657742430.2850129574161.2269230769231489.37138688751743440.2859122937164.85000000000002489.120610412000744450.2833374291168.47307692307695489.8386015017115445460.2819327781172.09615384615387490.230283036466146470.280950271175.71923076923082490.504251347005447480.2783426854179.3423076923077491.23136656125748490.2760378863182.96538461538464491.874050945442849500.2750515975186.58846153846156492.149073773810550510.2733032783190.21153846153848492.636585862923751520.2705773895193.8346153846154493.3966894604872752530.2693867429197.45769230769233493.728696701306553540.2668428361201.08076923076925494.438055309785854550.2647137465204.7038461538462495.0317437712122355560.262707631208.32692307692312495.5911413565225456570.2608075472211.95000000000005496.1209724070157657580.258787752215.5730769230769496.6841845286703658590.2572638162219.19615384615386497.1091281658812659600.2545525987222.81923076923078497.865140740701360610.2524048203226.4423076923077498.464040491874561620.2505808633230.06538461538463498.97264388720462630.2484600892233.68846153846155499.564013579624263640.2463899614237.31153846153848500.141260755596364650.2444194603240.9346153846154500.690727404239365660.242879313244.55769230769232501.1201915767800366670.241473521248.18076923076927501.512191268997367680.2398388184251.80384615384617501.9680217825086368690.2383022049255.42692307692312502.3965005535393469700.2366533218259.05502.856285259521570710.235239394262.6730769230769503.25055359081871720.233660628266.29615384615386503.690786412275572730.2317111795269.91923076923075504.234382632271373740.2311872488273.5423076923077504.380478713100374750.2297222894277.1653846153846504.788977007378675760.2283891983280.78846153846155505.1607043263614476770.227418326284.41153846153844505.4314283276891677780.2263222822288.0346153846154505.737055932449778790.2249495566291.6576923076923506.1198351775765379800.2238570883295.28076923076924506.424465761339580810.2228417561298.9038461538462506.707587228715581820.222269031302.5269230769231506.867289434403582830.2214197429306.15507.1041101633350683840.2199927083309.7730769230769507.5020332550905784850.219465178313.3961538461539507.649133055631485860.2191108087317.01923076923083507.7479475769007486870.2184206627320.6423076923077507.9403921196294587880.2177433089324.2653846153847508.129269627311188890.2168089343327.88846153846157508.3898163857810589900.2169744997331.5115384615385508.34364911401990910.2159874364335.1346153846154508.618887932365391920.215664626338.75769230769237508.7089023543412692930.215100225342.38076923076926508.866283407034893940.2145543638346.0038461538462509.018494722051894950.2140555297349.6269230769231509.157592692335795960.2137871603353.25509.232426447753596970.2138504603356.87307692307695509.2147754898860597980.2136630712360.49615384615385509.267028228656898990.2130417717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base quality distribution on read startpositionfraction0-34-78-1112-1516-1920-2324-2728-3132-3536-3940-43>=44
Per base quality distribution on read 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base quality distribution on read endpositionfraction0-34-78-1112-1516-1920-2324-2728-3132-3536-3940-43>=44

Per position quality percentiles (approximation)

Shows the mean for all bases and the means of the lowest and highest percentiles to indicate the spread. Since the graph is based on the binned phreds, rather than exact phreds, it is an approximation.

Per position quality percentiles001010202030304040505013265105140186248332443592790105614111886252033674500601480371074114355191852564134269458006121181809109337146129195301261020348852466241Per position quality percentilespositionphred score1-54.93494952719.83003496592688494.6339488280076516-104.62642938524.96734972393903497.94675397004613611-155.4881096230.104664481951176488.694266609380631116-205.69045918235.24197923996333486.521492200815261621-265.5024539640.379293997975466488.54024099245892127-315.26656820345.51660875598761491.07311792067662732-365.61753853950.65392351399976487.30449417591073237-415.73244851655.7912382720119486.07062217963053742-465.82001621860.92855303002405485.13034407863674247-535.97277276466.0658677880362483.49008594477044754-586.15700098471.20318254604832481.51189356302735459-646.32902542376.34049730406049479.664742054501355965-706.50277259981.47781206207264477.799092262523356571-786.66657453186.61512682008478476.04023178897437179-856.80436225591.75244157809692474.56070478525137986-906.87806578996.88975633610907473.76929634247068691-996.935825816102.02707109412123473.149084919959991100-1046.972862346107.16438585213336472.75139676180527100105-1096.988521266112.3017006101455472.58325555450995105110-1147.003323537117.43901536815765472.42431280779647110115-1207.017711866122.5763301261698472.2698148487133115121-1267.024276989127.71364488418193472.199320352422121127-1327.03966523132.85095964219408472.0340856158713127133-1397.059608161137.98827440020625471.81994386710033133140-1457.080559831143.1255891582184471.59497054455755140146-1537.116666343148.26290391623053471.2072686620286146154-1607.160310692153.40021867424267470.73862755326434154161-1687.206636111158.5375334322548470.2411978348913161169-1767.251441464163.67484819026694469.7600901700615169177-1857.291322154168.8121629482791469.3318622021997177186-1947.321938985173.94947770629125469.0031070185049186195-2047.3400169179.08679246430336468.8089913007169195205-2147.347443027184.22410722231552468.72925156754116205215-2257.354642033189.3614219803277468.6519506076584215226-2367.352018048194.49873673833983468.68012624225753226237-2477.347055885199.636051496352468.73340860183066237248-2607.344528708204.77336625436413468.7605447321489248261-2737.349351927209.91068101237624468.70875432948503261274-2867.348798354215.0479957703884468.71469843676664274287-3007.360219224220.18531052840055468.5920642507869287301-3157.375714203225.3226252864127468.4256833956082301316-3317.397345235230.45994004442485468.1934152757749316332-3477.423915096235.597254802437467.90811535104933332348-3647.456172642240.73456956044913467.56174261702364348365-3827.473299952245.8718843184613467.37783424061325365383-4017.485011201251.00919907647346467.2520820392508383402-4217.487858432256.1465138344856467.2215092445207402422-4427.491440978261.28382859249774467.18304084308636422443-4647.496668017266.4211433505099467.1269143240604443465-4877.509648277271.5584581085221466.9875358335919465488-5117.532626981276.6957728665342466.7407967782774488512-5367.558167229281.8330876245463466.46655255753126512537-5637.582385658286.97040238255846466.2065016732388537564-5917.600878445292.10771714057057466.007931170569564592-6207.613800079297.24503189858274465.86918219401592621-6517.632493676302.3823466565949465.66845544043974621652-6837.658457155307.519661414607465.3896666823389652684-7177.681822265312.65697617261924465.13877850473256684718-7527.710714566317.7942909306314464.8285408591806718753-7897.729090886322.9316056886435464.631220944058753790-8287.748832603328.0689204466557464.419239776965790829-8707.769554025333.20623520466785464.19673879672894829871-9137.794510002338.3435499626799463.9287683192581871914-9587.814911151343.48086472069207463.70970634426055914959-10057.83703856348.61817947870423463.47210826024069591006-10557.857584685353.75549423671634463.2514895753178610061056-11077.882645324358.8928089947285462.982395274512210561108-11627.903060264364.0301237527407462.763185209908811081163-12207.927136962369.1674385107528462.50465619979811631221-12807.950642644374.30475326876496462.252258594805212211281-13437.973939705379.4420680267771462.002101107155712811344-14107.997844492384.57938278478923461.745418019739413441411-14808.019414016389.7166975428014461.513810353317114111481-15538.04215078394.85401230081357461.269669183038214811554-16308.064930574399.9913270588256461.02506596896615541631-17118.090818502405.1286418168378460.747088463178916311712-17968.112818996410.26595657484995460.510853151641117121797-18858.137892624415.4032713328621460.241619380277717971886-19788.160408383420.5405860908743459.999851298711518861979-20768.185330654425.67790084888645459.7322427501999319792077-21798.206897846430.81521560689856459.500660119443220772180-22878.232001137435.95253036491073459.2311078244796421802288-24008.253829694441.0898451229229458.996718735744722882401-25198.276655632446.227159880935458.751620037994524012520-26438.299275469451.3644746389472458.5087343946105425202644-27748.321321585456.5017893969593458.2720092236721726442775-29128.344376895461.6391041549714458.0244475843400727752913-30568.365087288466.77641891298356457.80206503650729133057-32078.383925628471.9137336709957457.5997840735426530573208-33668.403328815477.05104842900784457.3914379506107432083367-35338.421793417482.18836318702457.193170084506433673534-37088.441825986487.32567794503217456.9780658239594735343709-38928.461415566492.4629927030443456.767718255486237093893-40848.477749598497.6003074610564456.5923278800580638934085-42878.491967701502.7376222190685456.439657763980240854288-44998.507560816507.8749369770807456.272223150181342884500-47228.523190687513.0122517350928456.104393860647145004723-49568.53258344518.1495664931051456.003537031092447234957-52018.543407709523.2868812511172455.887308984671749575202-54598.556067404528.4241960091294455.7513726314466652025460-57298.567007527533.5615107671414455.633900580400354605730-60138.574516771538.6988255251537455.553268369623957306014-63118.581627974543.8361402831657455.476910202307560146312-66238.592824103548.9734550411779455.356689228932263126624-69518.597851646554.11076979919455.3027048396770666246952-72958.6027771559.2480845572022455.249816657645969527296-76578.60854917564.3853993152144455.187837746304972967658-80368.616172534569.5227140732264455.1059801422693576588037-84348.622547942574.6600288312387455.0375227489122480378435-88528.63098872579.7973435892508454.946887973955784358853-92908.638728493584.934658347263454.863780408071388539291-97508.645171456590.0719731052751454.794597629649492919751-102338.653393767595.2092878632873454.70630869753455975110234-107408.663917324600.3466026212993454.593309606808531023410741-112728.671024051605.4839173793115454.51699950830121074111273-118308.679383248610.6212321373237454.427240730518061127311831-124168.68655827615.7585468953358454.350197303428051183112417-130318.693912796620.895861653348454.27122640915921241713032-136778.70265974626.03317641136454.177304108435751303213678-143548.708492211631.1704911693722454.11467663215971367814355-150658.717383658636.3078059273844454.019202694512051435515066-158118.724134163641.4451206853965453.94671761591161506615812-165958.728332475646.5824354434087453.90163728145151581216596-174168.736591634651.7197502014209453.81295269066941659617417-182798.741261035656.8570649594329453.76281392986351741718280-191848.747657551661.9943797174451453.694129890651651828019185-201358.754646398667.1316944754573453.61908555353941918520136-211328.764008772672.2690092334694453.518554932527762013621133-221798.772034722677.4063239914816453.43237447757092113322180-232778.778188528682.5436387494938453.3662965867122218023278-244308.786596523687.6809535075058453.276013825651832327824431-256408.795577542692.818268265518453.1795780954762443125641-269108.803678575697.9555830235302453.092591415791332564126911-282438.811348543703.0928977815423453.01023338367462691128244-296428.820590362708.2302125395545452.91099725664712824429643-311108.829804705713.3675272975667452.812056152946642964331111-326518.838170542718.5048420555786452.722226073863343111132652-342688.845356264723.6421568135909452.64506775675353265234269-359658.854832965728.7794715716029452.543309524782963426935966-377478.864044838733.9167863296152452.444394947235373596637748-396168.875311791739.0541010876273452.323413472538733774839617-415788.884367156744.1914158456395452.22617943580843961741579-436388.896518656749.3287306036515452.095699937884664157943639-457998.909139283754.4660453616638451.96018308872884363945800-480688.921548998759.6033601196759451.82693095423394580048069-504488.938637201764.7406748776881451.643442497723134806950449-529478.951104607769.8779896357003451.509570890538445044952948-555698.968581254775.0153043937124451.32191141516415294855570-583228.979680797780.1526191517245451.202727557132735557058323-612108.995175418785.2899339097365451.03635054084175832361211-642429.015470704790.4272486677488450.818425291654656121164243-674249.036156524795.5645634257609450.59630660306216424367425-707649.058981398800.7018781837731450.35121932144656742570765-742699.074927273805.8391929417852450.17999686664787076574270-779479.099129603810.9765076997974449.920118849159057427077948-818089.116062287816.1138224578094449.73830030649737794881809-858609.137075109821.2511372158217449.512670358833258180985861-901129.167820333826.3884519738338449.182536526956148586190113-945769.187806811831.525766731846448.967927178275439011394577-992609.221284129836.6630814898581448.60845686739749457799261-1041769.261094783841.8003962478701448.1809809153171599261104177-1093369.289466256846.9377110058823447.8763357822558104177109337-1147529.322854204852.0750257638945447.5178250999678109337114753-1204359.374760582857.2123405219066446.96046856655005114753120436-1264009.409763047862.3496552799188446.58462164444927120436126401-1326619.436751127867.4869700379311446.294831009065126401132662-1392329.491903008872.624284795943445.70262514927936132662139233-1461289.553814002877.7615995539553445.03784177498716139233146129-1533669.56733362882.8989143119674444.8926718049062146129153367-1609629.606952991888.0362290699796444.4672498039266153367160963-1689349.644452541893.1735438279917444.0645898623284160963168935-1773029.628725159898.3108585860039444.23346620889527168935177303-1860839.595023654903.4481733440159444.59534377472505177303186084-1953009.629034335908.5854881020281444.23014636214805186084195301-2049739.654843573913.7228028600402443.95301379557725195301204974-2151269.663741276918.8601176180524443.85747269368176204974215127-2257819.465638369923.9974323760646445.98464768267024215127225782-2369649.41465861929.1347471340767446.53205442658646225782236965-2487009.153527912934.2720618920888449.3360046415894236965248701-2610199.390927156939.409376650101446.786876303732248701261020-2739479.026459502944.5466914081131450.70043057226246261020273948-2875158.648413258949.6840061661253454.7597880384892273948287516-3017568.279936948954.8213209241374458.7163861612494287516301757-3167027.924092338959.9586356821496462.5373485420315301757316703-3323887.672328295965.0959504401617465.2407221709831316703332389-3488517.341094061970.2332651981739468.7974250394526332389348852-3661307.552393578975.370579956186466.52854845163915348852366131-3842647.221163598980.5078947141982470.08520564515635366131384265-4032977.471652349985.6452094722104467.3955257446828384265403298-4232727.488123249990.7825242302224467.2186657183659403298423273-4442377.870743947995.9198389882346463.1101890152838423273444238-4662406.9351720961001.0571537462466473.15610439041575444238466241-48933310.185355681006.194468504259438.25651945672416466241489334-51356912.63620091011.3317832622711411.94001187477073489334top 1%top 5%meanbottom 5%bottom 1%
Per position quality percentiles on read start00101020203030404050501112131415161718191Per position quality percentiles on read startpositionphred score6.4956540016.669230769230769496.394628447941914.53659723210.037529137529138518.245646262891624.18647239413.405827505827506522.150884834570535.37558575916.774125874125875508.887697306169344.46524796620.14242424242424519.041464998264154.81548493323.51072261072261515.134975742490765.11585872126.87902097902098511.7846527317621573.95625757230.247319347319348524.718665543561484.2696346633.615617715617724521.22330571204995.10002726236.983916083916085511.9612343870376105.12941651440.352214452214454511.63343119405386115.54591540143.720512820512816506.9878666756398125.61834257547.08881118881119506.18002512042204135.33655060450.457109557109554509.3230894185102145.84580779853.82540792540792503.64291302474567155.93917377457.19370629370629502.6015232894259165.489512460.56200466200467507.6169770739085175.66675078863.93030303030303505.64008736985903185.85503853267.29860139860139503.53995483454037195.51913978470.66689976689976507.2865177947473205.58703516774.03519813519813506.5292231417779215.60092984477.40349650349651506.37424404294626225.40427329780.77179487179485508.56772091400165235.49895294484.14009324009325507.51167869884904245.53464309487.50839160839162507.11359625727624255.39128936290.87668997668999508.71254173561016265.39528521694.24498834498836508.6679725885929275.1734369697.61328671328671511.142433903176285.02200803100.98158508158508512.8314488978157295.266414856104.34988344988346510.1053727644741305.493434668107.71818181818182507.5732287081821315.515109699111.0864801864802507.33146873709563325.599590862114.45477855477857506.3891788443882335.664073911117.82307692307693505.6699448376162345.634102085121.1913752913753506.00424597686003355.679708054124.55967365967368505.4955640158954365.721470219127.92797202797205505.02975524601237375.698809254131.29627039627042505.28251216683736385.7325901134.66456876456874504.90572581104203395.761954384138.03286713286715504.5782011058768405.748788182141.4011655011655504.72505489686654415.777646147144.76946386946386504.40317759125287425.809697012148.13776223776225504.04568717445807435.807796202151.50606060606063504.0668885135569445.841556258154.87435897435896503.69033404441336455.866003134158.24265734265734503.4176573516495465.882257758161.61095571095572503.23635577732307475.91880592164.9792540792541502.82870320334837485.952518797168.34755244755246502.45267496159204495.971222025171.7158508158508502.2440620325351505.999337683175.0841491841492501.93046430035577516.036203095178.45244755244755501.5192731710084526.059870197181.82074592074594501.25529395214676536.094995534185.1890442890443500.86351135162045546.126451585188.55734265734264500.51265539998155556.159295301191.92564102564106500.14632164167494566.189429419195.29393939393938499.81021032752426576.218679497198.66223776223777499.4839594545196586.244164549202.03053613053615499.19970310789284596.284753301205.39883449883447498.746982408896606.317703621208.76713286713286498.3794596080735616.344776526212.13543123543124498.0774925992316626.37910497215.50372960372962497.69459840828114636.410058955218.87202797202798497.3493424247491646.440287707222.24032634032636497.0121755799099656.467898467225.60862470862472496.7042094050828666.490649644228.97692307692307496.4504462772983676.515348854232.34522144522145496.1749550937393686.539724233235.7135198135198495.90307586368505696.566517513239.08181818181822495.6042277413893706.589264262242.45011655011655495.3505140017171716.615703924245.81841491841493495.0556100836941726.64354069249.18671328671329494.74512307373715736.658073192252.55501165501164494.5830297772615746.678136602255.92331002331002494.3592455975281756.700031236259.2916083916084494.11503621203065766.717621201262.65990675990673493.9188404478016776.735885194266.0282051282051493.7151266811822786.755360437269.3965034965035493.49790281258703796.775952827272.7648018648019493.2682184675544806.791300062276.1331002331002493.09703777026044816.802731154279.50139860139865492.9695371290207826.816112043282.869696969697492.8202887523744836.840323708286.2379953379953492.550235562234846.850805904289.60629370629374492.4333187606481856.856864253292.97459207459207492.36574486827686866.867100402296.34289044289045492.2515724349416876.878763985299.71118881118883492.12147863187545886.894084593303.0794871794872491.9505949290115896.893847564306.44778554778554491.95323870751486906.908046985309.8160839160839491.79486054943425916.914108463313.1843822843823491.7272517588933926.925380773316.55268065268064491.6015221493447936.932227379319.9209790209791491.5251561533727946.940736971323.2892773892774491.4302414785169956.947765848326.6575757575757491.3518424656658966.945513402330.02587412587417491.3769658989577976.949622006333.3941724941725491.3311391674261986.959834707336.7624708624708491.21722827016646996.961440766340.1307692307692491.1993145367502100top 1%top 5%meanbottom 5%bottom 1%
Per position quality percentiles on read end0010102020303040405050-100-90-80-70-60-50-40-30-20-10Per position quality percentiles on read endpositionphred score7.0608220966.669230769230769490.09083046943937-1007.04980797310.037529137529138490.2136802980809-997.04606156213.405827505827506490.2554671931308-987.03794277516.774125874125875490.3460228970764-977.03772921120.14242424242424490.34840495454733-967.03189077223.51072261072261490.413526001428-957.01928419926.87902097902098490.55413777897786-947.02327226930.247319347319348490.5096554624796-937.01446948133.615617715617724490.6078404092832-927.00853243536.983916083916085490.6740613042231-917.00197403840.352214452214454490.74721265241726-906.99287287743.720512820512816490.8487255974542-896.98554037747.08881118881119490.93051117408856-886.98487590250.457109557109554490.93792263351156-876.97008467353.82540792540792491.1029017238071-866.96428577457.19370629370629491.167581755095-856.95020447760.56200466200467491.32464237595514-846.93994798563.93030303030303491.43904171109386-836.93647845267.29860139860139491.4777403435662-826.92987684270.66689976689976491.5513736872578-816.92103854174.03519813519813491.64995473644024-806.90499552677.40349650349651491.8288960588236-796.89303919480.77179487179485491.96225514213177-786.88060233784.14009324009325492.1009739329678-776.86641184787.50839160839162492.25925248070166-766.85240447390.87668997668999492.41548857434344-756.84346975494.24498834498836492.5151450498588-746.82769039897.61328671328671492.6911455574035-736.812393253100.98158508158508492.86176755878574-726.79782083104.34988344988346493.02430612413457-716.775996789107.71818181818182493.2677281234033-706.751519615111.0864801864802493.5407427600162-696.746780168114.45477855477857493.5936058233176-686.721655123117.82307692307693493.8738467035519-676.707925506121.1913752913753494.02698474141187-666.686855656124.55967365967368494.2619946092978-656.666627377127.92797202797205494.4876177211793-646.65115346131.29627039627042494.6602114035147-636.630038178134.66456876456874494.89572801924436-626.604802061138.03286713286715495.1772077772156-616.577820209141.4011655011655495.4781592044254-606.556774023144.76946386946386495.712905125609-596.532283266148.13776223776225495.9860712598805-586.513947218151.50606060606063496.19058872351513-576.489665295154.87435897435896496.4614255559403-566.465658152158.24265734265734496.72919753343103-556.444900237161.61095571095572496.96072812160173-546.421875524164.9792540792541497.2175422302581-536.393026478168.34755244755246497.53932004914327-526.368213638171.7158508158508497.8160786548128-516.340649606175.0841491841492498.12352363065463-506.321896442178.45244755244755498.33269353625104-496.289841158181.82074592074594498.69023324069127-486.269891896185.1890442890443498.9127442352787-476.250991531188.55734265734264499.1235559964667-466.223238804191.92564102564106499.4331056528065-456.193881054195.29393939393938499.76055747166237-446.175115192198.66223776223777499.9698690088121-436.156249089202.03053613053615500.1802986256339-426.132185592205.39883449883447500.4486991614414-416.105963238208.76713286713286500.7411792723077-406.082364057212.13543123543124501.00440090691836-396.058188273215.50372960372962501.2740538825168-386.038628424218.87202797202798501.49222141942715-376.00976818222.24032634032636501.81412414616875-365.983798381225.60862470862472502.10378728392135-355.97063563228.97692307692307502.2506025860596-345.947817271232.34522144522145502.5051150538161-335.924980319235.7135198135198502.75983490537044-325.915270006239.08181818181822502.86814224467673-315.885207132242.45011655011655503.2034589148357-305.86279554245.81841491841493503.45343436109613-295.855089114249.18671328671329503.5393906493065-285.832918397252.55501165501164503.7866794126346-275.807498378255.92331002331002504.07021040210896-265.809149481259.2916083916084504.0517942498104-255.793798472262.65990675990673504.22301704549363-245.768664243266.0282051282051504.5033603707069-235.766565426269.3965034965035504.52677025081215-225.729211924272.7648018648019504.9434054610419-215.67795665276.1331002331002505.515098908072-205.66145629279.50139860139865505.69914137989133-195.596579085282.869696969697506.42277174971724-185.496579053286.2379953379953507.5381567181007-175.409927914289.60629370629374508.5046501936423-165.336339425292.97459207459207509.32544487003105-155.255292451296.34289044289045510.22943035195067-145.269250424299.71118881118883510.07374526749413-135.208542011303.0794871794872510.7508775665378-124.994421813306.44778554778554513.1391413201208-114.933630671309.8160839160839513.8171963627819-104.762510259313.1843822843823515.7258471145277-94.613481436316.55268065268064517.3880916754104-84.425946514319.9209790209791519.4798273419203-73.932776096323.2892773892774524.9805743139199-63.093987111326.6575757575757534.3362976095207-53.329263951330.02587412587417531.7120559334311-42.674250025333.3941724941725539.0179804934082-31.856115092336.7624708624708548.143331671411-21.412728608340.1307692307692553.0887962990606-1top 1%top 5%meanbottom 5%bottom 1%

Per sequence average quality scores

>=Q5 1,403,578 48.38%
>=Q7 1,199,332 41.34%
>=Q10 757,656 26.12%
>=Q12 210,743 7.26%
>=Q15 28 0.00%
>=Q20 0 0.00%
>=Q30 0 0.00%
Per sequence quality scores00224466881010121214141616181820202222036912151821242730333639035.080300187617254568.846153846153800.135540076561.656285178236395567.174025256432716.95266817888.23227016885554483.07259127433593222.60282449114.80825515947467289.99999999999994321.92619271141.38424015009383298.347459839174844.116943647167.9602251407129518.056311087361652.923625649194.53621013133207532.778016967057763.69563876221.11219512195123523.253858295797874.923910379247.68818011257034508.100937159240886.605475998274.2641651031895487.3558260545167498.873979787300.8401500938087459.3697857796042109.978672776327.4161350844279445.74142745946375116.174760521353.9921200750469492.6694696782617121.052055731380.5681050656661555.867169980872130.03674611434407.1440900562853568.3928249863769140.0009651887444433.72007504690436568.8342465215068150460.29606003752355568.8461538461538160486.8720450281426568.8461538461538170513.4480300187618568.8461538461538180540.0240150093808568.8461538461538190566.5999999999999568.8461538461538200593.1759849906191568.8461538461538210619.7519699812382568.8461538461538220646.3279549718575568.8461538461538230672.9039399624764568.8461538461538240699.4799249530956568.8461538461538250726.0559099437148568.8461538461538260752.6318949343339568.8461538461538270779.2078799249531568.8461538461538280805.783864915572568.8461538461538290832.3598499061914568.8461538461538300858.9358348968104568.8461538461538310885.5118198874295568.8461538461538320912.0878048780488568.8461538461538330938.6637898686678568.8461538461538340965.239774859287568.8461538461538350991.8157598499062568.84615384615383601018.3917448405252568.84615384615383701044.9677298311444568.84615384615383801071.5437148217636568.846153846153839Per sequence quality scoresPhred scorePercentage of total

Per position base content

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Per position N content

Per position N content000.10.10.20.20.30.30.40.40.50.50.60.60.70.70.80.80.90.911132651051401862483324435927901056141118862520336745006014803710741143551918525641342694580061211818091093371461291953012610203488524662411-5023.870344785088758547.624090954578816-10029.32019519264327547.6240909545788611-15034.770045600197776547.62409095457881116-20040.21989600775229547.62409095457881621-26045.6697464153068547.62409095457882127-31051.1195968228613547.62409095457882732-36056.56944723041582547.62409095457883237-41062.01929763797033547.62409095457883742-46067.46914804552483547.62409095457884247-53072.91899845307934547.62409095457884754-58078.36884886063385547.62409095457885459-64083.81869926818837547.62409095457885965-70089.26854967574288547.62409095457886571-78094.71840008329738547.62409095457887179-850100.16825049085189547.62409095457887986-900105.61810089840641547.62409095457888691-990111.0679513059609547.624090954578891100-1040116.51780171351542547.6240909545788100105-1090121.96765212106995547.6240909545788105110-1140127.41750252862444547.6240909545788110115-1200132.86735293617897547.6240909545788115121-1260138.3172033437335547.6240909545788121127-1320143.767053751288547.6240909545788127133-1390149.21690415884248547.6240909545788133140-1450154.66675456639703547.6240909545788140146-1530160.11660497395152547.6240909545788146154-1600165.56645538150602547.6240909545788154161-1680171.01630578906054547.6240909545788161169-1760176.46615619661503547.6240909545788169177-1850181.91600660416955547.6240909545788177186-1940187.3658570117241547.6240909545788186195-2040192.81570741927857547.6240909545788195205-2140198.2655578268331547.6240909545788205215-2250203.7154082343876547.6240909545788215226-2360209.1652586419421547.6240909545788226237-2470214.61510904949662547.6240909545788237248-2600220.06495945705115547.6240909545788248261-2730225.51480986460564547.6240909545788261274-2860230.96466027216016547.6240909545788274287-3000236.41451067971468547.6240909545788287301-3150241.86436108726915547.6240909545788301316-3310247.31421149482367547.6240909545788316332-3470252.76406190237822547.6240909545788332348-3640258.21391230993265547.6240909545788348365-3820263.6637627174872547.6240909545788365383-4010269.11361312504175547.6240909545788383402-4210274.56346353259624547.6240909545788402422-4420280.01331394015074547.6240909545788422443-4640285.4631643477053547.6240909545788443465-4870290.9130147552598547.6240909545788465488-5110296.3628651628143547.6240909545788488512-5360301.8127155703688547.6240909545788512537-5630307.2625659779233547.6240909545788537564-5910312.7124163854778547.6240909545788564592-6200318.16226679303236547.6240909545788592621-6510323.61211720058685547.6240909545788621652-6830329.06196760814134547.6240909545788652684-7170334.5118180156959547.6240909545788684718-7520339.9616684232504547.6240909545788718753-7890345.4115188308049547.6240909545788753790-8280350.86136923835943547.6240909545788790829-8700356.3112196459139547.6240909545788829871-9130361.76107005346836547.6240909545788871914-9580367.21092046102297547.6240909545788914959-10050372.66077086857746547.62409095457889591006-10550378.11062127613195547.624090954578810061056-11070383.5604716836865547.624090954578810561108-11620389.01032209124094547.624090954578811081163-12200394.4601724987955547.624090954578811631221-12800399.91002290635004547.624090954578812211281-13430405.35987331390453547.624090954578812811344-14100410.809723721459547.624090954578813441411-14800416.2595741290135547.624090954578814111481-15530421.70942453656806547.624090954578814811554-16300427.1592749441225547.624090954578815541631-17110432.6091253516771547.624090954578816311712-17960438.0589757592316547.624090954578817121797-18850443.50882616678604547.624090954578817971886-19780448.95867657434064547.624090954578818861979-20760454.4085269818951547.624090954578819792077-21790459.8583773894496547.624090954578820772180-22870465.3082277970042547.624090954578821802288-24000470.7580782045586547.624090954578822882401-25190476.20792861211316547.624090954578824012520-26430481.65777901966766547.624090954578825202644-27740487.10762942722215547.624090954578826442775-29120492.55747983477664547.624090954578827752913-30560498.00733024233125547.624090954578829133057-32070503.45718064988574547.624090954578830573208-33660508.9070310574402547.624090954578832083367-35330514.3568814649948547.624090954578833673534-37080519.8067318725492547.624090954578835343709-38920525.2565822801037547.624090954578837093893-40840530.7064326876583547.624090954578838934085-42870536.1562830952126547.624090954578840854288-44990541.6061335027672547.624090954578842884500-47220547.0559839103219547.624090954578845004723-49560552.5058343178763547.624090954578847234957-52010557.9556847254307547.624090954578849575202-54590563.4055351329853547.624090954578852025460-57290568.8553855405398547.624090954578854605730-60130574.3052359480944547.624090954578857306014-63110579.7550863556488547.624090954578860146312-66230585.2049367632034547.624090954578863126624-6951059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position N contentpositionfractionN

Per sequence GC content

For short reads with fixed size (i.e. Illumina) the plot will look very spiky due to the GC content calculation: GC bases / all bases. For read lengths of 151, both 75 and 76 GC bases lead to a percentage of 50% (rounded) and 72 and 73 GC bases leads to 48% (rounded). Only 74 GC bases leads to 49%. As a result the even categories will be twice as high, which creates a spike. The smoothened plot is provided to give a clearer picture in this case.

Per sequence GC content001000010000200002000030000300004000040000500005000060000600007000070000800008000090000900001000001000001100001100001200001200001300001300000369121518212427303336394245485154576063666972757881848790939699026.38035034272658568.84615384615380136.848743335872044568.84410483261971847.31713632901751568.82976173788012657.78552932216298568.83385976494843568.25392231530844568.83590877848274778.72231530845391568.831810751414251189.19070830159939568.823614697277361099.65910129474486568.8256637108116719110.12749428789033568.8072225890035817120.59588728103577568.811320616072921131.06428027418124568.8031245619351023141.53267326732671568.79902653486661127152.00106626047216568.79083048072971243162.46945925361763568.75804626418213101172.93785224676313568.63920347919671481183.40624523990857568.68018374988131588193.87463823305404568.665840655141716170204.3430312261995568.497821545334917170214.81142421934499568.497821545334918239225.27981721249046568.356439611473119407235.74821020563593568.012205337722720654246.21660319878137567.5060989947681212054256.68499619192687564.6374800468475222099267.15338918507234564.5452744378072232403277.6217821782178563.9223743234015243027288.0901751713633562.6437898780428253528298.55856816450876561.617234097394264564309.0269611576542559.4944560759327276351319.4953541507997555.8328688902656289574329.9637471439451549.22889826944562914506340.4321401370906539.12316351862823021754350.90053313023606524.27191342253653133932361.3689261233815499.3190266026963251848371.837319116527462.608900123449533374386382.30571210967247416.428233088996763499942392.7741051028179364.063643208240835118923403.2424980959634325.171317315040536128410413.7108910891089305.732325915810137136088424.17928408225436290.038134553434.6476770753998293.1452357750414439122366445.1160700685453318.116563716690140105820455.58446306169077352.01954165404294192466466.0528560548362379.382068390136744281776476.5212490479817401.286023071044554372932486.9896420411272419.40749876776574463753497.45803503427265438.215393998453464555955507.9264280274181454.193601538371074650250518.3948210205637465.883223751147454743068528.863214013709480.599238953984834619539.3316070068545497.91135430468074939297549.8488.326068991557465026171560.2683929931454515.22142064184715128930570.7367859862909509.568192300909355226254581.2051789794364515.05135251850615323864591.6735719725818519.94849486531345425231602.1419649657274517.14749336402245528536612.6103579588728510.375503633395575630506623.0787509520183506.338946970964465736707633.5471439451636493.633014045210535843551644.0155369383092479.60956541694745947951654.4839299314546470.593905866339876058200664.9523229246001449.593566154027036164105675.4207159177455437.49414123440496293172685.889108910891377.935464834971076385516696.3575019040366393.622712453028166492211706.8258948971819379.904566841365076578230717.2942878903275408.551825063420556697546727.7626808834729368.97307963625356778913738.2310738766184407.15234881954216866641748.6994668697638432.29784291160026954967759.1678598629093456.218026910189347047656769.6362528560547471.198364858937457132742780.1046458492002501.757352708428477230079790.5730388423457507.213875750080267323813801.0414318354912520.0529945555597417682811.5098248286366532.61549653391697514869821.9782178217821538.37937160570317610118832.4466108149276548.114234906825778001842.9150038080732552.4519965587878785703853.3833968012185557.1606296604459793851863.8517897943641560.955402725838802670874.3201827875095563.3752877097625811592884.788575780655565.5841242996613821145895.2569687738003566.500033349461783681905.7253617669459567.450775629343984347916.1937547600912568.135146149776385230926.6621477532367568.374880733281186111937.1305407463823568.61871334385448777947.5989337395276568.68837980401838820958.0673267326732568.80517357546938920968.5357197258186568.80517357546939014979.0041127189642568.8174676566746913989.4725057121095568.8400068055512922999.9408987052552568.84205581908539311010.4092916984005568.84410483261979421020.877684691546568.84205581908539521031.3460776846912568.84205581908539601041.8144706778367568.84615384615389711052.2828636709824568.84410483261979801062.7512566641278568.84615384615389911073.219649657273568.8441048326197100Per sequence GC contentGC %number of reads
Per sequence GC content (smoothened)200002000040000400006000060000800008000010000010000012000012000014000014000016000016000018000018000020000020000022000022000024000024000026000026000006121824303642485460667278849096Per sequence GC content (smoothened)GC %number of reads121.146153846153844568.846031831495301442.29230769230769568.819346763873821263.43846153846154568.823452158892542184.58461538461538568.8049778813083636105.73076923076923568.7741874186681844126.87692307692308568.75776583859341070148.02307692307687568.704395703350312182169.16923076923075568.474493582303614258190.31538461538457568.318488571593216409211.46153846153842568.0085312476818181061232.60769230769228566.6701724715876204153253.75384615384615560.3232317726874225430274.9557.7019370532515248092296.0461538461538552.23765628337042615925317.1923076923077536.15887669265692836260338.3384615384615494.417272840089773085780359.4846153846153392.767692177236332174328380.6307692307692211.005433119596134247333401.77692307692361.1482514497330436270641422.923076923076913.30397790187794338228186444.0692307692307100.4512506611962440174242465.21538461538455211.181965105399942136685486.36153846153843288.275125463937644106205507.5076923076923350.841345548828034677687528.6538461538461409.380173120379944865468549.8434.46208398708855055184570.9461538461538455.572025173210935249095592.0923076923076468.07090030762365459042613.2384615384616447.652718182151035680258634.3846153846152404.1026878238493558106151655.5307692307691350.9521912143327460157277676.676923076923246.0059783514683462177727697.8230769230769204.0283142853263664175776718.9692307692306208.033127126061966145554740.1153846153846270.0697512535408668102623761.2615384615383358.194108027308237062821782.4076923076923439.895574294329447241495803.553846153846483.671401378659257424987824.6999999999999517.55733186295627613704845.8461538461538540.7179178609161786521866.9923076923077555.4624440705542802737888.1384615384613563.2298514459225821028909.2846153846153566.737911489396184341930.4307692307691568.14811467831748697951.576923076923568.6489728705988834972.7230769230769568.7782928136869905993.8692307692307568.83782104145799231015.0153846153846568.84192643647669421036.1615384615384568.8439791339869611057.3076923076922568.84603183149539811078.453846153846568.8460318314953100

Adapter content

Only adapters that are present more than 0.1% are shown. Given the 12 bp length of the sequences used to estimate the content, values below this threshold are problably false positives. The legend is sorted from most frequent to least frequent.

For nanopore the adapter mix (AMX) and ligation kit have overlapping adapter sequences and are therefore indistinguishable. Please consult the nanopore documentation for more information which adapters are used by your kit.

For illumina short reads, the last part of the graph will be flat as the 12 bp probes cannot be found in the last 11 base pairs.

Adapter counts are accumulated towards the start for front (5') adapters and towards the end for end (3') adapters.

Adapter content (%)0010102020303040405050606070708080909010010013265105140186248332443592790105614111886252033674500601480371074114355191852564134269458006121181809109337146129195301261020348852466241Adapter content (%)position%1-520.6516609721.753111889003804303.774892844739916-1020.6475933927.38863310377163303.7900822024916611-1520.6349769933.02415431853946303.837194956195841116-2019.7451763838.659675533307286307.159931326055131621-263.25551269344.29519674807511368.736429140915332127-310.401690872849.930717962842934379.393308284148532732-360.154188901955.56623917761076380.31754208632533237-410.108618204861.20176039237858380.487714382492133742-460.0603587675566.83728160714641380.667927101579554247-530.0325751201272.47280282191424380.771678138425564754-580.0254396176278.10832403668205380.798323876176375459-640.0242676027283.74384525144988380.80270047078285965-700.022819819689.37936646621772380.808106852355376571-780.0221303990795.01488768098555380.810681319770947179-850.02147544956100.65040889575336380.813127063815677986-900.02116521032106.28593011052119380.81428557415278691-990.02106179724111.92145132528901380.81467174426591100-1040.02082050006117.55697254005683380.8155728078604100105-1090.02064814492123.19249375482467380.8162164247143105110-1140.02057920287128.8280149695925380.81647387145586110115-1200.02054473184134.4635361843603380.81660259482663115121-1260.02026896363140.09905739912813380.81763238179286121127-1320.02020002158145.734578613896380.8178898285344127133-1390.02006213747151.3700998286638380.81840472201753133140-1450.01971742721157.0056210434316380.81969195572526140146-1530.01971742721162.6411422581994380.81969195572526146154-1600.01951060105168.27666347296727380.82046429594993154161-1680.01951060105173.91218468773508380.82046429594993161169-1760.01947613002179.54770590250288380.8205930193207169177-1850.01940718797185.18322711727072380.82085046606227177186-1940.01937271694190.81874833203855380.82097918943305186195-2040.01933824591196.45426954680636380.8211079128038195205-2140.01930377489202.08979076157422380.8212366361746205215-2250.01926930386207.72531197634203380.8213653595454215226-2360.01926930386213.36083319110986380.8213653595454226237-2470.01926930386218.9963544058777380.8213653595454237248-2600.01920036181224.6318756206455380.82162280628694248261-2730.0190624777230.2673968354133380.82213769977005261274-2860.01899353565235.90291805018117380.8223951465116274287-3000.01899353565241.53843926494898380.8223951465116287301-3150.01895906462247.17396047971678380.8225238698824301316-3310.01895906462252.80948169448462380.8225238698824316332-3470.01889012257258.4450029092525380.82278131662395332348-3640.01882118052264.08052412402026380.8230387633655348365-3820.01882118052269.71604533878815380.8230387633655365383-4010.01878670949275.351566553556380.8231674867363383402-4210.01875223846280.98708776832376380.82329621010706402422-4420.01861435436286.6226089830916380.8238111035901422443-4640.01847647025292.25813019785943380.82432599707323443465-4870.0184075282297.89365141262726380.8245834438148465488-5110.0184075282303.52917262739504380.8245834438148488512-5360.01833858614309.16469384216293380.82484089055635512537-5630.01826964409314.80021505693077380.8250983372979537564-5910.01820070204320.43573627169854380.82535578403946564592-6200.01816623101326.0712574864664380.82548450741024592621-6510.01813175998331.7067787012342380.825613230781621652-6830.01809728896337.342299916002380.8257419541518652684-7170.0180283469342.9778211307698380.82599940089335684718-7520.0180283469348.6133423455377380.82599940089335718753-7890.01792493382354.2488635603055380.8263855710057753790-8280.01775257869359.88438477507333380.8270291878596790829-8700.01771810766365.51990598984116380.82715791123036829871-9130.01768363664371.155427204609380.82728663460114871914-9580.01747681048376.79094841937683380.82805897482575914959-10050.01740786843382.42646963414467380.82831642156739591006-10550.01730445535388.06199084891244380.8287025916796410061056-11070.01716657124393.69751206368034380.8292174851627610561108-11620.01702868713399.33303327844817380.829732378645911081163-12200.01675291892404.96855449321595380.830762165612111631221-12800.01668397687410.6040757079838380.8310196123536612211281-13430.01658056379416.2395969227516380.83140578246612811344-14100.01637373763421.8751181375194380.832178122690613441411-14800.01627032455427.5106393522872380.8325642928029414111481-15530.01602902736433.14616056705506380.833465356398414811554-16300.01585667223438.7816817818229380.834108973252315541631-17110.01585667223444.41720299659073380.834108973252316311712-17960.01578773018450.05272421135857380.8343664199938417121797-18850.01561537504455.68824542612634380.8350100368477317971886-19780.01551196196461.3237666408942380.8353962069600718861979-20760.0153051358466.959287855662380.836168547184719792077-21790.0151672517472.5948090704298380.836683440667820772180-22870.01506383862478.2303302851976380.8370696107801321802288-24000.01496042554483.8658514999655380.8374557808924722882401-25190.01482254143489.5013727147333380.837970674375624012520-26430.01468465733495.1368939295011380.838485567858725202644-27740.01458124425500.772415144269380.8388717379710326442775-29120.0143744180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nanopore ligation kit or Adapter Mix (AMX), top strandOxford nanopore ligation kit or Adapter Mix (AMX), bottom strand Oxford nanopore Native Adapter (NA), top strandOxford nanopore VN primerOxford nanopore Strand Switching Primer II (SSPII)Oxford nanopore RT Primer (RTP)Oxford nanpore cDNA primer, forward sequence

For the reads start and end plots, only adapters found in the first and last 100 bp are shown. All adapter counts are accumulated towards the start and end respectively.

Adapter content (%) on read start001010202030304040505060607070808090901001001112131415161718191Adapter content (%) on read startposition%120.630909418.592307692307692414.66940396227481220.6308404712.93185703185703414.669755301584252320.6304612917.27140637140637414.671687667786053420.6302199921.610955710955714414.672917355369074520.6298063425.95050505050505414.67502539122565620.6268418330.290054390054387414.690132981530846720.6252906334.62960372960374414.6980381159937820.6241530938.96915306915307414.70383521459858920.6220158943.308702408702416414.714726733190791020.6182585447.64825174825175414.7338747255543101120.6142254351.98780108780109414.75442807515566111220.597851756.32735042735042414.83787116114405121320.4535215160.66689976689976415.57340000542524131420.317188665.00644910644911416.2681734898131141520.1080528869.34599844599845417.33396128495366151619.7244248373.68554778554778419.2889888722488161718.7688879778.02509712509713424.15855170087866171817.4775688482.36464646464646430.7393126360628181912.2807168786.7041958041958457.223269780515819206.27438178891.04374514374513487.8324774265819320213.23476113595.38329448329448503.3228519094734621222.02014004299.72284382284381509.512747862922322231.405176928104.06239316239315512.646694502992923241.034337624108.4019425019425514.536548648389724250.7399895277112.74149184149185516.036591829979625260.530267802117.08104118104119517.105366009250226270.3809393148121.42059052059054517.866366953464827280.282627947125.76013986013986518.367376808704828290.2192012581130.09968919968918518.690608973375729300.1786633308134.43923853923854518.897196487317530310.1527411188138.77878787878788519.029300067661331320.1334373439143.11833721833722519.127675074300332330.1208898902147.45788655788655519.191618828615733340.1100315169151.7974358974359519.2469547698534350.102378949156.13698523698525519.285953433196335360.09510556235160.4765345765346519.323019730340536370.08786664677164.81608391608393519.359910357830237380.07914547704169.1556332556333519.404354780472438390.06928676344173.4951825951826519.454596301720239400.0592556947177.83473193473193519.505716171241640410.04919015494182.1742812742813519.557011710417641420.03960720955186.51383061383063519.605847874427742430.03078262674190.85337995337997519.65081930603443440.02330241397195.1929292929293519.688939621106744450.01792493382199.53247863247864519.716344087241945460.01433994706203.87202797202798519.734613731331946470.01182356212208.21157731157732519.747437616125947480.00978977155212.55112665112665519.75780212575448490.008307517407216.89067599067602519.765355920906649500.007273386609221.23022533022532519.77062601054850510.006273726838225.5697746697747519.775720430534651520.005480893227229.909324009324519.779760832593152530.004998298855234.2488733488734519.78222020775953540.004688059616238.5884226884227519.783801234651454550.004446762429242.927972027972519.785030922234555560.004067581137247.2675213675214519.786963288436356570.003929697031251.6070707070707519.787665967055157580.003653928818255.94662004662007519.789071324292858590.003516044712260.2861693861694519.789774002911659600.003136863419264.6257187257187519.791706369113560610.00282662418268.96526806526805519.793287396005961620.002481913914273.3048174048174519.79504409255362630.002378500834277.6443667443668519.795571101517163640.002171674675281.9839160839161519.796625119445564650.002068261595286.32346542346545519.797152128409665660.001826964409290.66301476301476519.798381815992666670.001689080303295.00256410256407519.799084494611467680.001551196196299.34211344211343519.799787173230268690.001447783117303.68166278166274519.800314182194469700.001447783117308.02121212121216519.800314182194470710.001378841063312.36076146076147519.800665521503871720.001240956957316.7003108003108519.801368200122672730.00120648593321.03986013986014519.801543869777373740.001103072851325.37940947940945519.802070878741574750.001034130798329.7189588189588519.802422218050975760.0009307177178334.0585081585081519.802949227015176770.0007928336115338.3980574980575519.80365190563477780.0007238915583342.73760683760685519.804003244943478790.0007238915583347.07715617715616519.804003244943479800.0006894205317351.4167055167055519.804178914598180810.0006894205317355.7562548562548519.804178914598181820.0005515364254360.0958041958042519.804881593216982830.0005170653988364.4353535353535519.805057262871683840.000413652319368.77490287490286519.805584271835884850.000413652319373.1144522144522519.805584271835885860.000413652319377.4540015540016519.805584271835886870.000413652319381.79355089355096519.805584271835887880.0003791812924386.13310023310027519.805759941490588890.0003447102659390.4726495726496519.805935611145289900.0003447102659394.81219891219894519.805935611145290910.0003102392393399.15174825174824519.806111280799991920.0002412971861403.4912975912976519.806462620109392930.0002412971861407.830846930847519.806462620109393940.0001723551329412.17039627039634519.806813959418794950.0001723551329416.50994560994565519.806813959418795960.0001723551329420.84949494949495519.806813959418796970.0001378841063425.1890442890443519.806989629073497980.0001034130798429.5285936285936519.807165298728198996.894205317e-05433.86814296814293519.8073409683828991006.894205317e-05438.20769230769235519.807340968382810010.34367613518.592307692307692518.0562658502089120.34364166412.93185703185703518.0564415198636230.34364166417.27140637140637518.0564415198636340.34364166421.610955710955714518.0564415198636450.343538250925.95050505050505518.0569685288277560.343469308930.290054390054387518.0573198681371670.343365895834.62960372960374518.0578468771013780.343331424838.96915306915307518.0580225467561890.343228011743.308702408702416518.05854955572029100.343159069747.64825174825175518.058900895029610110.342814359451.98780108780109518.060657591576711120.340504800656.32735042735042518.072427458442512130.338229712960.66689976689976518.084021655653513140.333989776665.00644910644911518.105629023183214150.327612636769.34599844599845518.138127909304915160.312238558873.68554778554778518.216476575306716170.287247064578.02509712509713518.343837074973217180.21778794682.36464646464646518.697811429218918190.126336312486.7041958041958519.163863023171119200.074940011891.04374514374513519.425786478347420210.0468461251395.38329448329448519.56895724693821220.0323338229499.72284382284381519.64291417157222230.02354371116104.06239316239315519.687709933523723240.01706315816108.4019425019425519.720735828609624250.01227168546112.74149184149185519.745153910614725260.008893524859117.08104118104119519.762369536776526270.006480552998121.42059052059054519.774666412606427280.005411951174125.76013986013986519.780112171902528290.004412291403130.09968919968918519.785206591889229300.003895226004134.43923853923854519.787841636709830310.003378160605138.77878787878788519.790476681530431320.003240276499143.11833721833722519.791179360149432330.003102392393147.45788655788655519.791882038768233340.00293003726151.7974358974359519.792760387041834350.002688740074156.13698523698525519.793990074624735360.002550855967160.4765345765346519.794692753243636370.002378500834164.81608391608393519.795571101517137380.002137203648169.1556332556333519.796800789100238390.001861435436173.4951825951826519.798206146337939400.001585667223177.83473193473193519.799611503575540410.001172014904182.1742812742813519.80171953943241420.000999659771186.51383061383063519.802597887705742430.0008962466912190.85337995337997519.803124896669843440.0006894205317195.1929292929293519.804178914598144450.0006549495051199.53247863247864519.804354584252845460.0004825943722203.87202797202798519.805232932526346470.0004481233456208.21157731157732519.80540860218147480.000413652319212.55112665112665519.805584271835848490.0003791812924216.89067599067602519.805759941490549500.0003102392393221.23022533022532519.806111280799950510.0003102392393225.5697746697747519.806111280799951520.0002757682127229.909324009324519.806286950454652530.0002412971861234.2488733488734519.806462620109353540.0002412971861238.5884226884227519.806462620109354550.0002412971861242.927972027972519.806462620109355560.0002412971861247.2675213675214519.806462620109356570.0002412971861251.6070707070707519.806462620109357580.0002412971861255.94662004662007519.806462620109358590.0002068261595260.2861693861694519.80663828976459600.0002068261595264.6257187257187519.80663828976460610.0002068261595268.96526806526805519.80663828976461620.0001378841063273.3048174048174519.806989629073462630.0001378841063277.6443667443668519.806989629073463640.0001034130798281.9839160839161519.807165298728164650.0001034130798286.32346542346545519.807165298728165660.0001034130798290.66301476301476519.807165298728166670.0001034130798295.00256410256407519.807165298728167686.894205317e-05299.34211344211343519.807340968382868696.894205317e-05303.68166278166274519.807340968382869706.894205317e-05308.02121212121216519.807340968382870716.894205317e-05312.36076146076147519.807340968382871726.894205317e-05316.7003108003108519.807340968382872736.894205317e-05321.03986013986014519.807340968382873746.894205317e-05325.37940947940945519.807340968382874756.894205317e-05329.7189588189588519.807340968382875766.894205317e-05334.0585081585081519.807340968382876776.894205317e-05338.3980574980575519.807340968382877786.894205317e-05342.73760683760685519.807340968382878796.894205317e-05347.07715617715616519.807340968382879806.894205317e-05351.4167055167055519.807340968382880816.894205317e-05355.7562548562548519.807340968382881826.894205317e-05360.0958041958042519.807340968382882836.894205317e-05364.4353535353535519.807340968382883846.894205317e-05368.77490287490286519.807340968382884856.894205317e-05373.1144522144522519.807340968382885866.894205317e-05377.4540015540016519.807340968382886873.447102659e-05381.79355089355096519.807516638037687883.447102659e-05386.13310023310027519.807516638037688893.447102659e-05390.4726495726496519.807516638037689903.447102659e-05394.81219891219894519.807516638037690913.447102659e-05399.15174825174824519.807516638037691923.447102659e-05403.4912975912976519.807516638037692933.447102659e-05407.830846930847519.807516638037693943.447102659e-05412.17039627039634519.807516638037694950416.50994560994565519.807692307692395960420.84949494949495519.807692307692396970425.1890442890443519.807692307692397980429.5285936285936519.807692307692398990433.86814296814293519.8076923076923991000438.20769230769235519.8076923076923100Oxford nanopore ligation kit or Adapter Mix (AMX), top strandOxford nanopore Native Adapter (NA), top strand
Adapter content (%) on read end00101020203030404050506060707080809090100100-100-90-80-70-60-50-40-30-20-10Adapter content (%) on read endposition%-1003.447102659e-058.592307692307692543.3459702216469-100-993.447102659e-0512.93185703185703543.3459702216469-99-983.447102659e-0517.27140637140637543.3459702216469-98-973.447102659e-0521.610955710955714543.3459702216469-97-963.447102659e-0525.95050505050505543.3459702216469-96-953.447102659e-0530.290054390054387543.3459702216469-95-946.894205317e-0534.62960372960374543.3457865971399-94-936.894205317e-0538.96915306915307543.3457865971399-93-926.894205317e-0543.308702408702416543.3457865971399-92-916.894205317e-0547.64825174825175543.3457865971399-91-906.894205317e-0551.98780108780109543.3457865971399-90-896.894205317e-0556.32735042735042543.3457865971399-89-886.894205317e-0560.66689976689976543.3457865971399-88-876.894205317e-0565.00644910644911543.3457865971399-87-860.000103413079869.34599844599845543.3456029726328-86-850.000103413079873.68554778554778543.3456029726328-85-840.000103413079878.02509712509713543.3456029726328-84-830.000103413079882.36464646464646543.3456029726328-83-820.000103413079886.7041958041958543.3456029726328-82-810.000103413079891.04374514374513543.3456029726328-81-800.000103413079895.38329448329448543.3456029726328-80-790.000103413079899.72284382284381543.3456029726328-79-780.0001378841063104.06239316239315543.3454193481258-78-770.0001378841063108.4019425019425543.3454193481258-77-760.0001378841063112.74149184149185543.3454193481258-76-750.0001378841063117.08104118104119543.3454193481258-75-740.0001378841063121.42059052059054543.3454193481258-74-730.0001723551329125.76013986013986543.3452357236189-73-720.0001723551329130.09968919968918543.3452357236189-72-710.0002068261595134.43923853923854543.3450520991119-71-700.0002068261595138.77878787878788543.3450520991119-70-690.0002068261595143.11833721833722543.3450520991119-69-680.0002068261595147.45788655788655543.3450520991119-68-670.0002412971861151.7974358974359543.3448684746048-67-660.0002757682127156.13698523698525543.3446848500978-66-650.0002757682127160.4765345765346543.3446848500978-65-640.0003447102659164.81608391608393543.3443176010838-64-630.000413652319169.1556332556333543.3439503520698-63-620.0004481233456173.4951825951826543.3437667275629-62-610.0004481233456177.83473193473193543.3437667275629-61-600.0005170653988182.1742812742813543.3433994785488-60-590.0005860074519186.51383061383063543.3430322295349-59-580.0007583625849190.85337995337997543.3421141069998-58-570.0007583625849195.1929292929293543.3421141069998-57-560.000827304638199.53247863247864543.3417468579859-56-550.0008617756646203.87202797202798543.3415632334788-55-540.0008617756646208.21157731157732543.3415632334788-54-530.0008962466912212.55112665112665543.3413796089718-53-520.0009307177178216.89067599067602543.3411959844648-52-510.0009307177178221.23022533022532543.3411959844648-51-500.0009651887444225.5697746697747543.3410123599579-50-490.0009651887444229.909324009324543.3410123599579-49-480.001103072851234.2488733488734543.3402778619298-48-470.001103072851238.5884226884227543.3402778619298-47-460.001275427984242.927972027972543.3393597393948-46-450.001551196196247.2675213675214543.3378907433388-45-440.001930377489251.6070707070707543.3358708737618-44-430.002654269047255.94662004662007543.3320147591147-43-420.004102052164260.2861693861694543.3243025298207-42-410.006652908131264.6257187257187543.3107143163026-41-400.01072048927268.96526806526805543.2890466244764-40-390.01454677322273.3048174048174543.2686643041993-39-380.02033790569277.6443667443668543.237815387023-38-370.02685292971281.9839160839161543.2031103551998-37-360.03440208453286.32346542346545543.1628965881665-36-350.04012427495290.66301476301476543.1324149200043-35-340.04301984118295.00256410256407543.1169904614162-34-330.04526045791299.34211344211343543.1050548684611-33-320.0461567046303.68166278166274543.100280631279-32-310.04663929897308.02121212121216543.097709888181-31-300.04691506718312.36076146076147543.096240892125-30-290.04715636437316.7003108003108543.094955520576-29-280.04725977745321.03986013986014543.0944046470551-28-270.04750107463325.37940947940945543.093119275506-27-260.04781131387329.7189588189588543.091466654943-26-250.04822496619334.0585081585081543.089263160859-25-240.04870756056338.3980574980575543.0866924177609-24-230.04963827828342.73760683760685543.0817345560719-23-220.05167206885347.07715617715616543.0709007101589-22-210.06384034124351.4167055167055543.0060812591873-21-200.0800072527355.7562548562548542.9199613654038-20-190.1349195981360.0958041958042542.6274475257505-19-180.3975543496364.4353535353535541.2284124069073-18-172.123242883368.77490287490286532.035802337406-17-167.533573918373.1144522144522503.21538509002414-16-158.836268484377.4540015540016496.27603134593636-15-149.067052007381.79355089355096495.04666527156235-14-139.729723022386.13310023310027491.51666774896853-13-129.729723022390.4726495726496491.51666774896853-12-119.729723022394.81219891219894491.51666774896853-11-109.729723022399.15174825174824491.51666774896853-10-99.729723022403.4912975912976491.51666774896853-9-89.729723022407.830846930847491.51666774896853-8-79.729723022412.17039627039634491.51666774896853-7-69.729723022416.50994560994565491.51666774896853-6-59.729723022420.84949494949495491.51666774896853-5-49.729723022425.1890442890443491.51666774896853-4-39.729723022429.5285936285936491.51666774896853-3-29.729723022433.86814296814293491.51666774896853-2-19.729723022438.20769230769235491.51666774896853-1Oxford nanopore ligation kit or Adapter Mix (AMX), bottom strand

Per tile quality

Per tile quality skipped. Reason: Can not parse header: '35eb0273-89e2-4093-98ed-d81cbdafcac7 runid=1d18d9e9682449156d70520e06571f01c4e6d2d8 sampleid=GM24185_1 read=41492 ch=2628 start_time=2019-01-26T18:52:46Z'.

Duplication percentages

Fingerprints are taken by taking a sample from the beginning and the end at an offset. The samples are combined and hashed while using the length as a seed. A subsample of these fingerprints is stored to estimate the duplication rate. See the documentation for a complete explanation.

Fingerprint front sequence length 8
Fingerprint front sequence offset 64
Fingerprint back sequence length 8
Fingerprint back sequence offset 0
Subsampled fingerprints 533,044
Estimated remaining sequences if deduplicated 72.71%
Duplication levels (%)00101020203030404050506060123456-1011-2021-3031-5051-100101-500501-10001001-50005001-1000010001-50000> 5000069.2727622453.60864984393376264.331666709181512.923519518118.98505209263345507.770198492997121.791596928184.36145434133314511.923276391475731.280608494249.73785659003286513.798117293825841.027842458315.1142588387325514.725527916371153.151868327380.4906610874322506.932375836688046-103.161416963445.86706333613193506.897341436483911-201.908090286511.24346558483165511.495856708985921-302.501060581576.6198678335314509.320220456312231-502.809208705641.9962700822311508.189610312583251-1005.133073883707.3726723309309499.66323828577174101-5001.849707198772.7490745796306511.71006704166234501-10003.189244416838.1254768283303506.795241184460561001-50000903.50187907703518.49673085262525001-100000968.8782813257296518.496730852625210001-5000001034.2546835744292518.4967308526252> 50000Duplication levels (%)Duplication countsPercentage of total

Overrepresented sequences

A subsample of the sequences is analysed Sequences are cut into fragments of up to 31 bp. Fragments are stored and counted. When the maximum amount of unique fragments is reached, only fragments that are already stored are counted. The rest of the fragments is ignored. Fragments are stored in their canonical representation. That is either the sequence or the reverse complement, whichever has the lowest sort order. Both representations are shown in the table. See the documentation for a complete explanation. The sequence identity is calculated by Number of matched nucleotides - gap sequences in query alignment Length of query .

The percentage shown is an estimate based on the number of occurences of the fragment in relation to the number of sampled sequences. Fragments are only counted once per sequence. Fragments that occur more than once in a sequence are counted as one.

Total sequences in file 2,900,987
Sampled sequences 362,624
Sampling rate 1 in 8
Total fragments sampled 2,600,688
Stored unique fragments 2,281,869
Maximum unique fragments 5,000,000
Fragment size 21
countpercentagecanonical sequencereverse complemented sequencesequence identitybest match
10768 2.97% GTAACTGAACGAAGTACAACA TGTTGTACTTCGTTCAGTTAC 85.71% Oxford nanopore Native Adapter (NA), bottom strand
7524 2.07% CTCCTCCTCCTCCTCCTCCTC GAGGAGGAGGAGGAGGAGGAG 71.43% gnl|uv|M97937.1:846-1007 Plasmid pGEX-5G/LIC expression vector(E. coli/S. japonicium) encoding glutathione S-transferase gene, complete cds
4976 1.37% TAACTGAACGAAGTACAATGA TCATTGTACTTCGTTCAGTTA 90.48% gnl|uv|NGB01107.1:1-36 Oxford Nanopore Technologies Ligation Sequencing Kit (SQK-LSK114) Ligation Adaptor Y Top
3122 0.86% GGAGGAGGAGGAGGAGGAGGA TCCTCCTCCTCCTCCTCCTCC 71.43% gnl|uv|M97937.1:846-1007 Plasmid pGEX-5G/LIC expression vector(E. coli/S. japonicium) encoding glutathione S-transferase gene, complete cds
2832 0.78% TAACTGAACGAAGTACACTGA TCAGTGTACTTCGTTCAGTTA 90.48% gnl|uv|NGB01107.1:1-36 Oxford Nanopore Technologies Ligation Sequencing Kit (SQK-LSK114) Ligation Adaptor Y Top
2356 0.65% GTAACTGAACGAAGCACAACA TGTTGTGCTTCGTTCAGTTAC 80.95% Oxford nanopore Native Adapter (NA), bottom strand
2258 0.62% CCTCCTCCTCCTCCTCCTCCA TGGAGGAGGAGGAGGAGGAGG 80.95% gnl|uv|FJ750577.1:368-2560 Cre-lox Univector acceptor vector pCR701
2233 0.62% TAACTGAACGAAGCATACTGA TCAGTATGCTTCGTTCAGTTA 80.95% Oxford nanopore Native Adapter (NA), bottom strand
2068 0.57% AGGAGGAGGAGGAGGAGGAGG CCTCCTCCTCCTCCTCCTCCT 71.43% gnl|uv|M97937.1:846-1007 Plasmid pGEX-5G/LIC expression vector(E. coli/S. japonicium) encoding glutathione S-transferase gene, complete cds
1843 0.51% TAACTGAACGAAGTATACTGA TCAGTATACTTCGTTCAGTTA 85.71% gnl|uv|NGB01107.1:1-36 Oxford Nanopore Technologies Ligation Sequencing Kit (SQK-LSK114) Ligation Adaptor Y Top
1260 0.35% TAACTGAACGAAGCATACCGA TCGGTATGCTTCGTTCAGTTA 85.71% gnl|uv|JX560321.2:370-1372 Cloning vector pSEVA111
1164 0.32% GTAACTGAACGAAGCATAACA TGTTATGCTTCGTTCAGTTAC 85.71% Oxford nanopore Native Adapter (NA), bottom strand
1076 0.30% TAACTGAACGAAGTAATAACA TGTTATTACTTCGTTCAGTTA 85.71% gnl|uv|NGB01098.1:1-61 Oxford Nanopore Technologies Sequencing Adapter Y_top
1042 0.29% CGCCTCCTCCTCCTCCTCCTC GAGGAGGAGGAGGAGGAGGCG 76.19% gnl|uv|M97937.1:846-1007 Plasmid pGEX-5G/LIC expression vector(E. coli/S. japonicium) encoding glutathione S-transferase gene, complete cds
999 0.28% TAACTGGAACGAAGTACAACA TGTTGTACTTCGTTCCAGTTA 80.95% Oxford nanopore Native Adapter (NA), bottom strand
976 0.27% GCAACTGAACGAAGTACAACA TGTTGTACTTCGTTCAGTTGC 80.95% Oxford nanopore Native Adapter (NA), bottom strand
940 0.26% AACTGAACGAAGTAATACTGA TCAGTATTACTTCGTTCAGTT 71.43% gnl|uv|NGB01098.1:1-61 Oxford Nanopore Technologies Sequencing Adapter Y_top
888 0.24% TAACTGAACGAAGCACAATGA TCATTGTGCTTCGTTCAGTTA 85.71% gnl|uv|NGB01107.1:1-36 Oxford Nanopore Technologies Ligation Sequencing Kit (SQK-LSK114) Ligation Adaptor Y Top
883 0.24% CTCCTCCTCCTCCTCCTCCAC GTGGAGGAGGAGGAGGAGGAG 71.43% gnl|uv|M97937.1:846-1007 Plasmid pGEX-5G/LIC expression vector(E. coli/S. japonicium) encoding glutathione S-transferase gene, complete cds
852 0.23% CACCTCCTCCTCCTCCTCCTC GAGGAGGAGGAGGAGGAGGTG 76.19% gnl|uv|M97937.1:846-1007 Plasmid pGEX-5G/LIC expression vector(E. coli/S. japonicium) encoding glutathione S-transferase gene, complete cds
849 0.23% CTCCTCCTCCTCCTCCTCCGC GCGGAGGAGGAGGAGGAGGAG 80.95% gnl|uv|NGB00972.1:1-45 Pacific Biosciences Blunt Adapter
822 0.23% TAACTGAACGAAGTATACCGA TCGGTATACTTCGTTCAGTTA 85.71% gnl|uv|NGB01107.1:1-36 Oxford Nanopore Technologies Ligation Sequencing Kit (SQK-LSK114) Ligation Adaptor Y Top
770 0.21% CTCCTCCTCCTCCTCCGCCTC GAGGCGGAGGAGGAGGAGGAG 80.95% gnl|uv|FJ750577.1:368-2560 Cre-lox Univector acceptor vector pCR701
745 0.21% AACTGAACGAAGTACAATTGA TCAATTGTACTTCGTTCAGTT 90.48% gnl|uv|NGB01098.1:1-61 Oxford Nanopore Technologies Sequencing Adapter Y_top
742 0.20% CTCCGCCTCCTCCTCCTCCTC GAGGAGGAGGAGGAGGCGGAG 80.95% gnl|uv|FJ750577.1:368-2560 Cre-lox Univector acceptor vector pCR701
739 0.20% GTAACTGAACGAAGTATAACA TGTTATACTTCGTTCAGTTAC 80.95% Oxford nanopore Native Adapter (NA), bottom strand
719 0.20% TAACTGAACGAAGCACACTGA TCAGTGTGCTTCGTTCAGTTA 85.71% gnl|uv|NGB01107.1:1-36 Oxford Nanopore Technologies Ligation Sequencing Kit (SQK-LSK114) Ligation Adaptor Y Top
709 0.20% CTCCTCCTCCTCCGCCTCCTC GAGGAGGCGGAGGAGGAGGAG 80.95% gnl|uv|M28248.1:1-1709 Retroviral vector pLXSN
709 0.20% CTCCTCCTCCACCTCCTCCTC GAGGAGGAGGTGGAGGAGGAG 71.43% gnl|uv|JN204911.1:1-169 Biobrick cloning vector BBa_96047
702 0.19% CTCCTCCGCCTCCTCCTCCTC GAGGAGGAGGAGGCGGAGGAG 85.71% gnl|uv|M28248.1:1-1709 Retroviral vector pLXSN
697 0.19% CTCCTCCTCCTCCACCTCCTC GAGGAGGTGGAGGAGGAGGAG 80.95% gnl|uv|JN204911.1:1-169 Biobrick cloning vector BBa_96047
693 0.19% CTCCACCTCCTCCTCCTCCTC GAGGAGGAGGAGGAGGTGGAG 76.19% gnl|uv|M97937.1:846-1007 Plasmid pGEX-5G/LIC expression vector(E. coli/S. japonicium) encoding glutathione S-transferase gene, complete cds
662 0.18% CTCCTCCTCCTCCTCCACCTC GAGGTGGAGGAGGAGGAGGAG 71.43% gnl|uv|FJ750577.1:368-2560 Cre-lox Univector acceptor vector pCR701
659 0.18% GTAACTGAACGAAGTACATCA TGATGTACTTCGTTCAGTTAC 95.24% gnl|uv|NGB01098.1:1-61 Oxford Nanopore Technologies Sequencing Adapter Y_top
649 0.18% CTCCTCCTCCGCCTCCTCCTC GAGGAGGAGGCGGAGGAGGAG 76.19% gnl|uv|M28248.1:1-1709 Retroviral vector pLXSN
639 0.18% CTCCTCCACCTCCTCCTCCTC GAGGAGGAGGAGGTGGAGGAG 66.67% gnl|uv|M97937.1:846-1007 Plasmid pGEX-5G/LIC expression vector(E. coli/S. japonicium) encoding glutathione S-transferase gene, complete cds
624 0.17% TAACTGAACGAAGTACACCGA TCGGTGTACTTCGTTCAGTTA 90.48% gnl|uv|NGB01107.1:1-36 Oxford Nanopore Technologies Ligation Sequencing Kit (SQK-LSK114) Ligation Adaptor Y Top
603 0.17% CCTCCTCCTCCTCCTCCTCCG CGGAGGAGGAGGAGGAGGAGG 80.95% gnl|uv|NGB00972.1:1-45 Pacific Biosciences Blunt Adapter
584 0.16% ATCCTCCTCCTCCTCCTCCTC GAGGAGGAGGAGGAGGAGGAT 71.43% gnl|uv|M97937.1:846-1007 Plasmid pGEX-5G/LIC expression vector(E. coli/S. japonicium) encoding glutathione S-transferase gene, complete cds
570 0.16% GATGGAGGAGGAGGAGGAGGA TCCTCCTCCTCCTCCTCCATC 71.43% gnl|uv|M97937.1:846-1007 Plasmid pGEX-5G/LIC expression vector(E. coli/S. japonicium) encoding glutathione S-transferase gene, complete cds
556 0.15% GAGGATGGAGGAGGAGGAGGA TCCTCCTCCTCCTCCATCCTC 71.43% gnl|uv|M97937.1:846-1007 Plasmid pGEX-5G/LIC expression vector(E. coli/S. japonicium) encoding glutathione S-transferase gene, complete cds
523 0.14% TAACTGAACGAAGTACAACGA TCGTTGTACTTCGTTCAGTTA 90.48% gnl|uv|NGB01107.1:1-36 Oxford Nanopore Technologies Ligation Sequencing Kit (SQK-LSK114) Ligation Adaptor Y Top
517 0.14% GTAACTGAACGAAGCATACCA TGGTATGCTTCGTTCAGTTAC 85.71% Oxford nanopore Native Adapter (NA), bottom strand
508 0.14% CAACTGAACGAAGTACAATGA TCATTGTACTTCGTTCAGTTG 85.71% gnl|uv|NGB01107.1:1-36 Oxford Nanopore Technologies Ligation Sequencing Kit (SQK-LSK114) Ligation Adaptor Y Top
506 0.14% AACTGAACGAAGTACAACAGA TCTGTTGTACTTCGTTCAGTT 76.19% Oxford nanopore Native Adapter (NA), bottom strand
500 0.14% AACTGGAACGAAGTACAATGA TCATTGTACTTCGTTCCAGTT 85.71% gnl|uv|NGB01107.1:1-36 Oxford Nanopore Technologies Ligation Sequencing Kit (SQK-LSK114) Ligation Adaptor Y Top
500 0.14% AACTGAACGAAGTATTACTGA TCAGTAATACTTCGTTCAGTT 71.43% gnl|uv|NGB01098.1:1-61 Oxford Nanopore Technologies Sequencing Adapter Y_top
499 0.14% CGTAACTGAACGAAGTACTGA TCAGTACTTCGTTCAGTTACG 90.48% Oxford nanopore Native Adapter (NA), bottom strand
490 0.14% GAGGAGGAGGAGGAGGATGGA TCCATCCTCCTCCTCCTCCTC 80.95% gnl|uv|M97937.1:846-1007 Plasmid pGEX-5G/LIC expression vector(E. coli/S. japonicium) encoding glutathione S-transferase gene, complete cds
485 0.13% ACGTAACTGAACGAAGTATGA TCATACTTCGTTCAGTTACGT 85.71% Oxford nanopore Native Adapter (NA), bottom strand
471 0.13% TAACTGAACGAAGTACATCGA TCGATGTACTTCGTTCAGTTA 90.48% gnl|uv|NGB01107.1:1-36 Oxford Nanopore Technologies Ligation Sequencing Kit (SQK-LSK114) Ligation Adaptor Y Top
470 0.13% GAGGAGGATGGAGGAGGAGGA TCCTCCTCCTCCATCCTCCTC 80.95% gnl|uv|U59231.1:8434-10310-48 Cloning vector cLHYGpk
453 0.12% GCCTCCTCCTCCTCCTCCTCC GGAGGAGGAGGAGGAGGAGGC 76.19% gnl|uv|M97937.1:846-1007 Plasmid pGEX-5G/LIC expression vector(E. coli/S. japonicium) encoding glutathione S-transferase gene, complete cds
452 0.12% GAGGAGGAGGATGGAGGAGGA TCCTCCTCCATCCTCCTCCTC 85.71% gnl|uv|U13843.1:1887-9923 pBPV cloning vector
449 0.12% GAGGAGGAGGAGGATGGAGGA TCCTCCATCCTCCTCCTCCTC 71.43% gnl|uv|M97937.1:846-1007 Plasmid pGEX-5G/LIC expression vector(E. coli/S. japonicium) encoding glutathione S-transferase gene, complete cds
445 0.12% GTAACTGAACGAAGTACACGA TCGTGTACTTCGTTCAGTTAC 95.24% gnl|uv|NGB01107.1:1-36 Oxford Nanopore Technologies Ligation Sequencing Kit (SQK-LSK114) Ligation Adaptor Y Top
444 0.12% CATTGTACTTCGTTCAGTTAC GTAACTGAACGAAGTACAATG 90.48% gnl|uv|NGB01098.1:1-61 Oxford Nanopore Technologies Sequencing Adapter Y_top
425 0.12% AACTGAACGAAGTAATACCGA TCGGTATTACTTCGTTCAGTT 71.43% gnl|uv|NGB01098.1:1-61 Oxford Nanopore Technologies Sequencing Adapter Y_top
402 0.11% GAGGAGGAGGAGGAGGATAAA TTTATCCTCCTCCTCCTCCTC 76.19% gnl|uv|U13843.1:1887-9923 pBPV cloning vector
392 0.11% GTAACCGAACGAAGTACAACA TGTTGTACTTCGTTCGGTTAC 80.95% Oxford nanopore Native Adapter (NA), bottom strand
382 0.11% AACTGAACGAAGTAATAATGA TCATTATTACTTCGTTCAGTT 76.19% gnl|uv|NGB01098.1:1-61 Oxford Nanopore Technologies Sequencing Adapter Y_top
381 0.11% AACTGAACGAAGTATTACCGA TCGGTAATACTTCGTTCAGTT 71.43% gnl|uv|NGB01098.1:1-61 Oxford Nanopore Technologies Sequencing Adapter Y_top
377 0.10% GTAACTGAACGAAGTATACCA TGGTATACTTCGTTCAGTTAC 80.95% Oxford nanopore Native Adapter (NA), bottom strand
371 0.10% ACGTAACTGAACGAAGTAACA TGTTACTTCGTTCAGTTACGT 95.24% Oxford nanopore Native Adapter (NA), bottom strand
368 0.10% ACAACTGAACGAAGTACAACA TGTTGTACTTCGTTCAGTTGT 76.19% Oxford nanopore Native Adapter (NA), bottom strand
366 0.10% GGAGGAGGAGGAGGAGGCGGA TCCGCCTCCTCCTCCTCCTCC 80.95% gnl|uv|FJ750577.1:368-2560 Cre-lox Univector acceptor vector pCR701

Nanopore time series

Base counts over time

Base count over time004000000040000000800000008000000012000000012000000016000000016000000020000000020000000024000000024000000028000000028000000032000000032000000000:0001:3003:0004:3006:0007:3009:0010:3012:0013:3015:0016:3018:0019:3021:0022:3024:0025:3027:0028:3030:0031:3033:0034:3036:0037:3039:0040:3042:0043:3045:0046:3000:00-00:1526545936423.155006774171937350.9652976639751500:0000:15-00:3031409262128.514962045970996313.8693400569729300:1500:30-00:4531785258133.874917317770056311.0013578290085500:3000:45-01:0031661619839.234872589569115311.9444328805934700:4501:00-01:1532036408044.59482786136817309.08566338078301:0001:15-01:3030819461749.95478313316723318.3681567072731601:1501:30-01:4535567607355.314738404966285282.1507575950824401:3001:45-02:0033949029660.67469367676535294.4967721223101:4502:00-02:1532214189466.03464894856441307.729601359743802:0002:15-02:3031301367271.39460422036346314.692329279432702:1502:30-02:4530674015276.75455949216251319.477578023243602:3002:45-03:0030580344082.11451476396157320.1920732223204602:4503:00-03:1534359345387.47447003576063291.367009743309303:0003:15-03:3033692963092.8344253075597296.4499696509413403:1503:30-03:4531934177598.19438057935875309.865446296441403:3003:45-04:00305380400103.55433585115782320.5147551662023503:4504:00-04:15302143535108.91429112295688322.983736563429904:0004:15-04:30292447496114.27424639475593330.379577884520904:1504:30-04:45310658899119.63420166655499316.488477939229704:3004:45-05:00315473010124.99415693835404312.81642164303304:4505:00-05:15289596930130.35411221015312332.553902246386305:0005:15-05:30281865570135.71406748195216338.451146652708405:1505:30-05:45278763121141.07402275375122340.8175996456035405:3005:45-06:00274198177146.4339780255503344.2995989923126705:4506:00-06:15273818668151.79393329734933344.5890768238195406:0006:15-06:30295580628157.1538885691484327.989720839800606:1506:30-06:45268782970162.51384384094746348.430152890957406:3006:45-07:00262002852167.87379911274652353.6018190596138306:4507:00-07:15258213179173.23375438454556356.492465453138307:0007:15-07:30253083442178.5937096563446360.405271586749107:1507:30-07:45247926271183.95366492814367364.339003534581907:3007:45-08:00317222240189.31362019994273311.482162620963107:4508:00-08:15273112183194.6735754717418345.1279619248108508:0008:15-08:30259475547200.03353074354084355.529569840112908:1508:30-08:45254835771205.3934860153399359.0686487424777608:3008:45-09:00247783905210.75344128713897364.4475959552104308:4509:00-09:15211068450216.11339655893804392.4530195319574709:0009:15-09:30312164005221.4733518307371315.340429220838409:1509:30-09:45251291070226.83330710253614361.7724380039187509:3009:45-10:00240106251232.1932623743352370.30387507106809:4510:00-10:15233096305237.55321764613427375.65084699284110:0010:15-10:30227562701242.91317291793334379.871710493674210:1510:30-10:45216540025248.2731281897324388.2794698508506610:3010:45-11:00271690701253.63308346153144346.212224819461710:4511:00-11:15233210100258.9930387333305375.5640476551801611:0011:15-11:30226040888264.3529940051295381.032502799519211:1511:30-11:45219712578269.7129492769286385.8595436757650511:3011:45-12:00216371677275.07290454872765388.407880544611611:4512:00-12:15210863132280.43285982052674392.6096298084181612:0012:15-12:30250077893285.7928150923258362.697812230074112:1512:30-12:45224757254291.1527703641249382.01161946544412:3012:45-13:00204058288296.5127256359239397.8001562119091512:4513:00-13:15200555604301.872680907723400.4718961937640513:0013:15-13:30197709920307.23263617952205402.642496715685213:1513:30-13:45191163031312.5925914513211407.6362629526811413:3013:45-14:00222935572317.9525467231202383.401142652127613:4514:00-14:15195475735323.31250199491916404.34666454565814:0014:15-14:30188309905328.67245726671825409.8125400040708514:1514:30-14:45181616658334.0324125385173414.9179436369222514:3014:45-15:00176972376339.3923678103164418.4604595779574714:4515:00-15:15172321685344.7523230821154422.007864107727415:0015:15-15:30204467505350.1122783539145397.488018028688915:1515:30-15:45197032920355.47223362571356403.158891562527215:3015:45-16:00173312373360.83218889751265421.252197670462815:4516:00-16:15167220487366.1921441693117425.898901566989616:0016:15-16:30160769835371.5520994411108430.819261170491516:1516:30-16:45161104789376.91205471290976430.563768527169316:3016:45-17:00178387819382.27200998470886417.3808030473835516:4517:00-17:15188298448387.6319652565079409.821279052461217:0017:15-17:30158507754392.991920528307432.5447072141238317:1517:30-17:45156229355398.35187580010603434.2826001279345317:3017:45-18:00151713554403.7118310719051437.7271147007022617:4518:00-18:15147886854409.07178634370416440.6460041547792318:0018:15-18:30119916867414.43174161550326461.980652829870118:1518:30-18:45206963808419.7916968873023395.5839146234361618:3018:45-19:00135591867425.1516521591014450.024243340582618:4519:00-19:15133053571430.51160743090037451.960377718957119:0019:15-19:30136591062435.87156270269946449.262088024496519:1519:30-19:45135711592441.2315179744985449.932920780703519:3019:45-20:00119073833446.5914732462976462.623693322207719:4520:00-20:15187247833451.95142851809663410.622655968288220:0020:15-20:30128033092457.31138378989573455.789845199427220:1520:30-20:45122487541462.67133906169477460.019821505629920:3020:45-21:00123009521468.03129433349386459.621671162713320:4521:00-21:15122007150473.3912496052929460.3862490342403421:0021:15-21:30117701217478.751204877092463.6706827300257621:1521:30-21:45154543603484.1111601488909435.5684400774966321:3021:45-22:00107553904489.47111542069471.41074202469721:4522:00-22:15106145989494.83107069248905472.4846564276386422:0022:15-22:30109232482500.1910259642882470.130374182418922:1522:30-22:45113544054505.5509812360872466.8416392302947322:3022:45-23:00111785283510.91093650788633468.183175834689222:4523:00-23:15140038049516.2708917796855446.632832006872923:0023:15-23:3095873260521.6308470514846480.320379202533723:1523:30-23:4589587522526.9908023232836485.114947462206723:3023:45-24:0094884969532.3507575950827481.074217281677323:4524:00-24:1599550598537.7107128668817477.515418503425624:0024:15-24:3098116478543.0706681386808478.609321277063724:1524:30-24:45122333071548.4306234104798460.137646486415324:3024:45-25:0088305447553.7905786822789486.0928749707220524:4525:00-25:1578057185559.150533954078493.9099350680429625:0025:15-25:3040974974564.5104892258769522.195108878834825:1525:30-25:4529811444569.870444497676530.710307349389625:3025:45-26:0029053780575.230399769475531.288230222709725:4526:00-26:1592643963580.5903550412742482.783587961355226:0026:15-26:30121120733585.9503103130731461.0623807490898726:1526:30-26:45120786503591.3102655848722461.3173211474059526:3026:45-27:00116566743596.6702208566712464.5360247203894326:4527:00-27:15114824173602.0301761284704465.86520369861927:0027:15-27:30110679204607.3901314002694469.0268589894899427:1527:30-27:45126634819612.7500866720685456.856404980340427:3027:45-28:00117742471618.1100419438675463.6392154434502727:4528:00-28:1591361148623.4699972156666483.7620799191861428:0028:15-28:3096563503628.8299524874657479.7938830012504428:1528:30-28:45100174982634.1899077592647477.039157528461728:3028:45-29:00105930659639.5498630310638472.648903550785628:4529:00-29:1595655635644.9098183028628480.486376881328329:0029:15-29:30135723066650.2697735746619449.9241687652342429:1529:30-29:4580283886655.6297288464609492.2114758038511629:3029:45-30:0086134948660.98968411826487.748465072163229:4530:00-30:1590237838666.3496393900591484.6189063525776530:0030:15-30:3094128539671.7095946618581481.6511988976251430:1530:30-30:4590224587677.0695499336572484.6290138091736730:3030:45-31:00120734668682.4295052054562461.356859296425230:4531:00-31:1569063364687.7894604772554500.7701460249395531:0031:15-31:3073190680693.1494157490544497.621955901323131:1531:30-31:4574527041698.5093710208535496.602620696122831:3031:45-32:0080093191703.8693262926524492.35693210417431:4532:00-32:1578899420709.2292815644516493.267504028412332:0032:15-32:30113197307714.5892368362506467.1061272125072632:1532:30-32:4554252562719.9491921080497512.067371771463332:3032:45-33:0059763807725.3091473798487507.863563030424132:4533:00-33:1563420585730.6691026516478505.074284869057733:0033:15-33:3066294105736.0290579234469502.882451899637833:1533:30-33:4563206522741.3890131952459505.2375655634440433:3033:45-34:00100032601746.748968467045477.147761390630433:4534:00-34:1547653756752.108923738844517.100738704564834:0034:15-34:3050702847757.4688790106431514.77498555840234:1534:30-34:4551883483762.8288342824421513.874432609515734:3034:45-35:0054685589768.1887895542412511.7370720501287534:4535:00-35:1556107114773.5487448260403510.652776356396135:0035:15-35:3087439921778.9087000978393486.753071671176635:1535:30-35:4540910737784.2686553696384522.244106893275935:3035:45-36:0045480738789.6286106414374518.75825022198735:4536:00-36:1546682166794.9885659132366517.841837772870836:0036:15-36:3049580489800.3485211850356515.631085835376836:1536:30-36:4549770656805.7084764568347515.486032277268436:3036:45-37:0072359339811.0684317286336498.256077331704836:4537:00-37:1539774605816.4283870004328523.110713555214337:0037:15-37:3033564119821.7883422722318527.847881896106637:1537:30-37:4536935425827.1482975440309525.276353025312837:3037:45-38:0036234584832.5082528158299525.810933056118637:4538:00-38:1539176224837.868208087629523.567140238889538:0038:15-38:3040794977843.2281633594281522.332405072700538:1538:30-38:4563353000848.5881186312271505.125836635267238:3038:45-39:0028176733853.9480739030262531.957214788792738:4539:00-39:1531901734859.3080291748253529.115898214374539:0039:15-39:3032272298864.6679844466244528.833243354662339:1539:30-39:4531745443870.0279397184233529.235112198137839:3039:45-40:0030892508875.3878949902224529.885704869764439:4540:00-40:1556405573880.7478502620215510.4251209803346740:0040:15-40:3021682343886.1078055338205536.91093639792440:1540:30-40:4523297742891.4677608056196535.678759560119540:3040:45-41:0024300656896.8277160774186534.913767504837940:4541:00-41:1524845894902.1876713492178534.497876672485741:0041:15-41:3024534868907.5476266210167534.73511777091141:1541:30-41:4557933820912.9075818928158509.2594210165212341:3041:45-42:0017659748918.2675371646147539.979248552919941:4542:00-42:1521757014923.627492436414536.85397964813342:0042:15-42:3020770656928.9874477082129537.606343294132142:1542:30-42:4521737531934.347402980012536.868840683289642:3042:45-43:0021995724939.707358251811536.671898977690442:4543:00-43:1555103715945.0673135236101511.4181383548102543:0043:15-43:3014156577950.4272687954092542.65136000344643:1543:30-43:4513505941955.7872240672082543.147645199265643:3043:45-44:0014450084961.1471793390073542.427481861183143:4544:00-44:1515246042966.5071346108064541.8203494985544:0044:15-44:3014896979971.8670898826055542.086604054566544:1544:30-44:4551014988977.2270451544044514.536893972156744:3044:45-45:009563612982.5870004262035546.15473290998344:4545:00-45:1511718397987.9469556980026544.511128966035645:0045:15-45:3011519213993.3069109698017544.663060415331545:1545:30-45:4511952196998.6668662416008544.332794255848345:3045:45-46:00121703521004.0268215133997544.166391546531345:4546:00-46:15442812401009.3867767851989519.673190526266646:0046:15-46:30133192101014.746732056998543.290077881903246:1546:30-46:4582321071020.1066873287971547.170364107241946:3046:45-47:0098255401025.4666426005958545.954942260878646:4547:00-47:15107404221030.826597872395545.25709831661247:0047:15-47:30106247851036.186553144194545.345302675407147:1547:30-47:4565489011041.5465084159935548.454262046050547:30Base count over timetime(HH:MM)base count

Read counts over time

Number of reads over time0010000100002000020000300003000040000400005000050000600006000070000700008000080000900009000000:0001:3003:0004:3006:0007:3009:0010:3012:0013:3015:0016:3018:0019:3021:0022:3024:0025:3027:0028:3030:0031:3033:0034:3036:0037:3039:0040:3042:0043:3045:0046:3000:00-00:159125423.885735731078896282.150757595082500:0000:15-00:304871529.4148412243842408.619513836507600:1500:30-00:453947934.9439467176895436.078208978513900:3000:45-01:003800640.4730522109948440.457448686631600:4501:00-01:153700346.0021577043001443.439374978038301:0001:15-01:303624551.531263197605405445.692914488393501:1501:30-01:454395857.0603686909107422.762109417984901:3001:45-02:003427362.589474184216451.5556848240405501:4502:00-02:153356768.11857967752131453.654627956376702:0002:15-02:303304873.64768517082662455.1976187292980302:1502:30-02:453147779.17679066413191459.868213149836302:3002:45-03:003154284.70589615743721459.6749676773124502:4503:00-03:154009890.23500165074252434.2379174786327703:0003:15-03:303237395.76410714404783457.204398636276603:1503:30-03:4531138101.29321263735311460.8760626142303:3003:45-04:0030442106.82231813065842462.945275673870103:4504:00-04:1529799112.35142362396373464.856919348221604:0004:15-04:3028628117.88052911726902468.338310860920704:1504:30-04:4535473123.40963461057432447.988076100523104:3004:45-05:0029934128.9387401038796464.4555633668258604:4505:00-05:1529139134.46784559718492466.819104146156205:0005:15-05:3027445139.99695109049023471.8553784608550405:1505:30-05:4527195145.52605658379554472.598630278254505:3005:45-06:0026539151.05516207710085474.5489230471107505:4506:00-06:1532693156.58426757040613456.2530363100052606:0006:15-06:3027418162.11337306371144471.935649657134206:1506:30-06:4524536167.64247855701672480.5038566081153706:3006:45-07:0023578173.17158405032205483.3519975723901306:4507:00-07:1523672178.70068954362733483.072534889047907:0007:15-07:3023273184.22979503693261484.258764789617507:1507:30-07:4529955189.75890053023792464.3931302141643307:3007:45-08:0033255195.28800602354326454.5822062244912407:4508:00-08:1526633200.81711151684854474.2694603637685308:0008:15-08:3022750206.34621701015382485.813647591617208:1508:30-08:4522121211.87532250345913487.6836691641942708:3008:45-09:0021616217.40442799676444489.1850378353412308:4509:00-09:1522798222.93353349006975485.670943242676509:0009:15-09:3033985228.46263898337503452.4119109176847409:1509:30-09:4528617233.9917444766803468.371013940886309:3009:45-10:0023508239.52084996998565483.56010808126209:4510:00-10:1521806245.04995546329093488.620166454117610:0010:15-10:3020582250.57906095659624492.2591273521054510:1510:30-10:4519973256.1081664499016494.069688779290610:3010:45-11:0031668261.6372719432068459.300368761343110:4511:00-11:1524145267.16637743651216481.666302450528111:0011:15-11:3020621272.69548292981744492.1431800685911611:1511:30-11:4519195278.2245884231228496.382688435037711:3011:45-12:0019389283.753693916428495.8059250247357611:4512:00-12:1518499289.28279940973334498.451901494677912:0012:15-12:3030396294.8119049030387463.0820340082716512:1512:30-12:4523972300.34101039634396482.1806327081685612:3012:45-13:0020088305.8701158896493493.7277929432868312:4513:00-13:1518348311.39922138295464498.9008255923871513:0013:15-13:3017421316.92832687625986501.6568033313044513:1513:30-13:4517292322.4574323695652502.0403212690825413:3013:45-14:0025781327.98653786287053476.8024625574659613:4514:00-14:1522074333.51564335617576487.823400505865414:0014:15-14:3018347339.0447488494811498.9037985996567414:1514:30-14:4516931344.5738543427863503.113576893407414:3014:45-15:0016834350.10295983609166503.401958598558414:4515:00-15:1516463355.632065329397504.504944295579215:0015:15-15:3024344361.1611708227023481.0746740038781615:1515:30-15:4521759366.6902763160076488.7598977957887315:3015:45-16:0017455372.21938180931295501.555721084138115:4516:00-16:1515458377.7484873026182507.492816601525116:0016:15-16:3015512383.2775927959235507.3322742089668516:1516:30-16:4515005388.80669828922873508.8395888946529316:3016:45-17:0021468394.3358037825341489.6250429112417416:4517:00-17:1520615399.8649092758394492.161018112208717:0017:15-17:3016867405.3940147691447503.3038493586616417:1517:30-17:4514458410.92312026244997510.46582387112317:3017:45-18:0013791416.4522257557553512.448819719944817:4518:00-18:1513659421.9813312490606512.841256679531718:0018:15-18:3015698427.5104367423659506.779294856821618:1518:30-18:4524244433.03954223567126481.371974730837918:3018:45-19:0019615438.5686477289765495.134025381806618:4519:00-19:1517362444.09775322228177501.832210760210719:0019:15-19:3015386449.62685871558705507.706873124936119:1519:30-19:4513264455.1559642088924514.015594551022919:3019:45-20:0012008460.6850697021977517.749691681637819:4520:00-20:1524728466.214175195503479.933039212352620:0020:15-20:3018943471.7432806888083497.131886266976420:1520:30-20:4516434477.2723861821136504.5911615063975520:3020:45-21:0014485482.8014916754189510.385552674843920:4521:00-21:1512518488.33059716872424516.23345797414321:0021:15-21:3011355493.8597026620296519.691065428685321:1521:30-21:4520885499.3888081553348491.358306149417321:3021:45-22:0017012504.9179136486401502.8727633045699621:4522:00-22:1514537510.44701914194536510.230956296824822:0022:15-22:3013318515.9761246352507513.855052158464622:1522:30-22:4512261521.5052301285559516.997520842429622:3022:45-23:0010726527.0343356218613521.561087001262422:4523:00-23:1518272532.5634411151665499.126774144876623:0023:15-23:3014344538.0925466084719510.8047466998571623:1523:30-23:4512996543.6216521017772514.812360499275223:3023:45-24:0012352549.1507575950824516.726977180896323:4524:00-24:1511370554.6798630883877519.646470319641424:0024:15-24:3010015560.2089685816931523.674895169946524:1524:30-24:4516105565.7380740749984505.5692808980952624:3024:45-25:0013248571.2671795683037514.063162667336424:4525:00-25:1511316576.796285061609519.807012712199625:0025:15-25:307812582.3253905549142530.224430184870725:1525:30-25:452175587.8544960482195546.98327216359425:3025:45-26:002067593.3836015415249547.304356948710625:4526:00-26:1510573598.9127070348302522.015957113510926:0026:15-26:3015935604.4418125281355506.074692133926926:1526:30-26:4513063609.9709180214408514.613169012212126:3026:45-27:0011297615.500023514746519.86349985032226:4527:00-27:1510184621.0291290080514523.172456941384427:0027:15-27:3010033626.5582345013567523.621381039093827:1527:30-27:4514668632.0873399946621509.8414923445074627:3027:45-28:0013034637.6164454879673514.699386223030427:4528:00-28:1511658643.1455509812724518.790244225997228:0028:15-28:3011135648.6746564745779520.345127027996828:1528:30-28:4510850654.2037619678831521.192434099832228:3028:45-29:0010160659.7328674611886523.243809115854828:4529:00-29:1510296665.2619729544938522.839480127189529:0029:15-29:3015684670.791078447799506.8209169585959429:1529:30-29:4510119676.3201839411043523.365702413908429:3029:45-30:0010260681.8492894344097522.94650838889529:4530:00-30:159519687.378394927715525.149506775667130:0030:15-30:309255692.9075004210202525.934380694840930:1530:30-30:458825698.4366059143257527.21277382076830:3030:45-31:0015552703.9657114076308507.2133539181828630:4531:00-31:158952709.4948169009361526.83520189752931:0031:15-31:307854715.0239223942415530.099563879547531:1531:30-31:457781720.5530278875468530.316593410228331:3031:45-32:007615726.0821333808522530.810112616981431:4532:00-32:157392731.6112388741574531.473093238101832:0032:15-32:3013779737.1403443674626512.4844958071832:1532:30-32:456834742.669449860768533.132031294537532:3032:45-33:006848748.1985553540733533.09040919276332:4533:00-33:156762753.7276608473786533.346087817948533:0033:15-33:306294759.2567663406838534.737455220120333:1533:30-33:455805764.7858718339892536.191255774953733:3033:45-34:0012034770.3149773272945517.672393492628333:4534:00-34:155551775.8440828205997536.946399621431534:0034:15-34:305348781.3731883139052537.549920097159934:1534:30-34:455582786.9022938072104536.854236396073934:3034:45-35:005268792.4313993005157537.787760678727834:4535:00-35:155208797.960504793821537.966141114903635:0035:15-35:3011057803.4896102871262520.577021595025535:1535:30-35:454928809.0187157804316538.798583150391135:3035:45-36:005457814.5478212737369537.225862304773735:4536:00-36:155268820.0769267670422537.787760678727836:0036:15-36:305091825.6060322603474538.313982965446536:1536:30-36:455402831.1351377536528537.389377704601736:3036:45-37:0010080836.6642432469581523.481649697422736:4537:00-37:154119842.1933487402634541.203746031495737:0037:15-37:303987847.7224542335688541.596182991082637:1537:30-37:453785853.251559726874542.196730459541437:3037:45-38:003755858.7806652201792542.285920677629337:4538:00-38:153605864.3097707134846542.73187176806938:0038:15-38:306100869.8388762067899535.314218630422338:1538:30-38:456541875.3679817000952534.003122424529738:3038:45-39:003143880.8970871934005544.105401126623338:4539:00-39:152943886.4261926867058544.700002580542839:0039:15-39:303065891.9552981800111544.337295693651939:1539:30-39:453016897.4844036733164544.482973049862339:3039:45-40:002797903.0135091666218545.134061641904239:4540:00-40:157752908.542614659927530.402810621046640:0040:15-40:302297914.0717201532323546.620565276703140:1540:30-40:452365919.6008256465376546.418400782370440:3040:45-41:002428925.1299311398428546.231101324385840:4541:00-41:152419930.6590366331483546.257858389812141:0041:15-41:302337936.1881421264535546.501644985919341:1541:30-41:457592941.7172476197588530.878491784182241:3041:45-42:001903947.2463531130641547.791930140924741:4542:00-42:152159952.7754586063694547.030840279907642:0042:15-42:302042958.3045640996747547.378682130450642:1542:30-42:451928963.8336695929801547.717604959184742:3042:45-43:001874969.3627750862854547.87814735174342:4543:00-43:157482974.8918805795905531.20552258383843:0043:15-43:301409980.420986072896549.26059573210643:1543:30-43:451468985.9500915662012549.085188303199743:3043:45-44:001427991.4791970595065549.207081601253343:4544:00-44:151395997.0083025528118549.302217833880444:0044:15-44:3012901002.5374080461171549.614383597188244:1544:30-44:4567251008.0665135394225533.456089086923644:3044:45-45:009651013.5956190327279550.580610959807544:4545:00-45:1511341019.124724526033550.078172731245445:0045:15-45:3011531024.6538300193383550.02168559312345:1545:30-45:459851030.1829355126436550.521150814415545:3045:45-46:0010151035.7120410059488550.431960596327645:4546:00-46:1563081041.2411464992542534.69583311834646:0046:15-46:3011141046.7702519925595550.137632876637446:1546:30-46:459191052.2993574858647550.71736929420946:3046:45-47:0010251057.82846297917550.402230523631746:4547:00-47:1510111063.3575684724754550.44385262540647:0047:15-47:309821068.8866739657806550.530069836224447:1547:30-47:456931074.415779459086551.389268937138147:30Number of reads over timetime(HH:MM)number of reads

Active channels over time

Active channels over time0020020040040060060080080010001000120012001400140016001600180018002000200022002200240024002600260000:0001:3003:0004:3006:0007:3009:0010:3012:0013:3015:0016:3018:0019:3021:0022:3024:0025:3027:0028:3030:0031:3033:0034:3036:0037:3039:0040:3042:0043:3045:0046:3000:00-00:15252024.068417970305646292.306327849944600:0000:15-00:30161729.63981101898751385.8826537697451600:1500:30-00:45152235.211204067669364395.7273392208869600:3000:45-01:00147740.782597116351226400.3906112766910600:4501:00-01:15145446.35399016503309402.7740614385463701:0001:15-01:30142651.92538321371495405.6756529399355601:1501:30-01:45261857.49677626239681282.150757595082501:3001:45-02:00156763.06816931107867391.06406716508301:4502:00-02:15146668.63956235976055401.5305222236653402:0002:15-02:30143374.2109554084424404.950255064588302:1502:30-02:45139679.78234845712427408.7845009771382402:3002:45-03:00138885.35374150580613409.613527120392302:4503:00-03:15258990.92513455448798285.155977364378403:0003:15-03:30161796.49652760316987385.8826537697451603:1503:30-03:451426102.06792065185171405.6756529399355603:3003:45-04:001385107.63931370053358409.924411924112603:4504:00-04:151363113.21070674921545412.204233818061204:0004:15-04:301338118.78209979789729414.794940515730104:1504:30-04:452498124.35349284657917294.586149743893204:3004:45-05:001583129.92488589526104389.4060148785748704:4505:00-05:151377135.49627894394288410.753438067366605:0005:15-05:301331141.06767199262472415.5203383910773605:1505:30-05:451305146.6390650413066418.2146733566530705:3005:45-06:001275152.21045808998846421.323521393855705:4506:00-06:152497157.7818511386703294.6897780117999406:0006:15-06:301613163.35324418735217386.2971668413722506:1506:30-06:451307168.92463723603402418.007416820839506:3006:45-07:001252174.49603028471589423.706971555711106:4507:00-07:151237180.06742333339776425.261395574312407:0007:15-07:301211185.63881638207963427.955730539888107:1507:30-07:452548191.2102094307615289.404736348555407:3007:45-08:001861196.78160247944334360.597356400496807:4508:00-08:151349202.3529955281252413.6550295687557708:0008:15-08:301252207.92438857680705423.706971555711108:1508:30-08:451205213.49578162548892428.5775001473286408:3008:45-09:001180219.0671746741708431.168206844997508:4509:00-09:151978224.63856772285266348.472849055406309:0009:15-09:302414230.20996077153453303.290924248060709:1509:30-09:451311235.78135382021637417.592903749212509:3009:45-10:001235241.35274686889824425.4686521101259609:4510:00-10:151171246.9241399175801432.1008612561583410:0010:15-10:301134252.49553296626195435.935107168708310:1510:30-10:451123258.0669260149439437.075018115682610:3010:45-11:002461263.6383190636257298.420395656443210:4511:00-11:151275269.20971211230756421.323521393855711:0011:15-11:301154274.78110516098945433.8625418105731311:1511:30-11:451113280.3524982096713438.1113007947501511:3011:45-12:001094285.9238912583532440.0802378849785411:4512:00-12:151076291.49528430703504441.9455467073001412:0012:15-12:302470297.0666773557169297.487741245282412:1512:30-12:451303302.6380704043988418.4219298924665512:3012:45-13:001133308.2094634530806436.0387354366150712:4513:00-13:151065313.7808565017625443.085457654274413:0013:15-13:301031319.35224955044436446.608818763104213:1513:30-13:451016324.92364259912625448.163242781705513:3013:45-14:002385330.4950356478081306.296144017356613:4514:00-14:151273336.06642869648994421.530777929669214:0014:15-14:301062341.63782174517183443.396342457994714:1514:30-14:451002347.2092147938537449.6140385324000614:3014:45-15:00970352.7806078425355452.930143105416314:4515:00-15:15948358.35200089121736455.2099649993648415:0015:15-15:302513363.92339393989926293.031725725291915:1515:30-15:451349369.4947869885811413.6550295687557715:3015:45-16:001040375.066180037263445.676164351943415:4516:00-16:15948380.6375730859449455.2099649993648416:0016:15-16:30924386.20896613462673457.69704342912716:1516:30-16:45900391.7803591833086460.1841218588891716:3016:45-17:002405397.3517522319904304.223578659221516:4517:00-17:151439402.9231452806723404.3284854571477417:0017:15-17:30992408.4945383293541450.650321211467617:1517:30-17:45903414.065931378036459.873237055168917:3017:45-18:00881419.63732442671784462.153058949117517:4518:00-18:15865425.20871747539974463.811111235625618:0018:15-18:302078430.78011052408164338.110022264730718:1518:30-18:452069436.3515035727635339.0426766758914718:3018:45-19:00968441.9228966214454453.1373996412297618:4519:00-19:15877447.49428967012716462.5675720207445419:0019:15-19:30850453.06568271880906465.36553525422719:1519:30-19:45826458.6370757674909467.852613683989119:3019:45-20:00790464.20846881617274471.5832313286323419:4520:00-20:152355469.7798618648546309.4049920545593320:0020:15-20:30937475.3512549135365456.349875946339220:1520:30-20:45842480.9226479622184466.1945613974810420:3020:45-21:00814486.4940410109002469.096152898870220:4521:00-21:15779492.0654340595821472.723142275606621:0021:15-21:30754497.636827108264475.313848973275521:1521:30-21:452292503.20822015694574315.933572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channels over timetime(HH:MM)active channels

Quality distribution over time

Quality distribution over 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5-35:000769.920404536117910.85195221519575234:4535:00-35:150775.292441966595810.85195221519563835:0035:15-35:300780.664479397073810.85195221519563835:1535:30-35:450786.036516827552110.85195221519563835:3035:45-36:000791.4085542580310.85195221519563835:4536:00-36:150796.780591688508110.85195221519563836:0036:15-36:300802.152629118986210.85195221519563836:1536:30-36:450807.524666549464310.85195221519563836:3036:45-37:000812.896703979942310.85195221519563836:4537:00-37:150818.268741410420510.85195221519563837:0037:15-37:300823.640778840898510.85195221519563837:1537:30-37:450829.012816271376710.85195221519575237:3037:45-38:000834.384853701854710.85195221519563837:4538:00-38:150839.756891132332610.85195221519575238:0038:15-38:300845.128928562810910.85195221519563838:1538:30-38:450850.500965993288910.85195221519563838:3038:45-39:000855.87300342376710.85195221519563838:4539:00-39:150861.24504085424510.85195221519575239:0039:15-39:300866.617078284723210.85195221519563839:1539:30-39:450871.989115715201310.85195221519563839:3039:45-40:000877.361153145679310.85195221519563839:4540:00-40:150882.733190576157310.85195221519563840:0040:15-40:300888.105228006635510.85195221519563840:1540:30-40:450893.477265437113510.85195221519575240:3040:45-41:000898.849302867591510.85195221519563840:4541:00-41:150904.221340298069710.85195221519563841:0041:15-41:300909.593377728547710.85195221519563841:1541:30-41:450914.965415159025810.85195221519563841:3041:45-42:000920.337452589503910.85195221519563841:4542:00-42:150925.709490019981910.85195221519563842:0042:15-42:300931.0815274504610.85195221519563842:1542:30-42:450936.453564880938210.85195221519563842:3042:45-43:000941.825602311416110.85195221519563842:4543:00-43:150947.197639741894210.85195221519563843:0043:15-43:300952.569677172372410.85195221519563843:1543:30-43:450957.941714602850310.85195221519563843:3043:45-44:000963.313752033328510.85195221519563843:4544:00-44:150968.685789463806610.85195221519563844:0044:15-44:300974.057826894284710.85195221519563844:1544:30-44:450979.429864324762710.85195221519563844:3044:45-45:000984.801901755240810.85195221519563844:4545:00-45:150990.17393918571910.85195221519563845:0045:15-45:300995.545976616196910.85195221519563845:1545:30-45:4501000.91801404667510.85195221519575245:3045:45-46:0001006.290051477152910.85195221519563845:4546:00-46:1501011.662088907631210.85195221519575246:0046:15-46:3001017.034126338109110.85195221519563846:1546:30-46:4501022.406163768587210.85195221519563846:3046:45-47:0001027.778201199065510.85195221519563846:4547:00-47:1501033.150238629543310.85195221519552447:0047:15-47:3001038.522276060021310.85195221519563847:1547:30-47:4501043.894313490499810.85195221519563847:30Quality distribution over timetime(HH:MM)fraction0-34-78-1112-1516-1920-2324-2728-3132-3536-3940-43>=44

Per channel base yield versus quality

Channel base yield and quality334455667788991010111112121010202030304040Channel base yield and qualitybase yield (megabases)quality (phred score)1721.955585: 8.526566459582.0024385203269241.167108123560981814.5216: 8.089227087392.3186233518964264.106579003385261912.692634: 9.488908435345.6511564827349190.69002519501552014.407952: 9.435163745389.4188076774954193.50905961251422411.14748: 8.701710656306.22536994391874231.980386183837372514.859154: 8.183460513400.9315719313413259.16381588197512617.905214: 7.345114749478.654123433927303.137008052252952721.664527: 6.271483527574.5758709559193359.451471708296942825.008158: 8.967931629659.8911742011418218.01647470254812298.700875: 9.9049466243.7983714215707168.8678522959869305.970407: 8.573875896174.12839126485608238.68561773017086347.340362: 9.545423898209.08384127034668187.725657214558963525.213738: 8.65460245665.1367051945895234.45132150856966365.126243: 8.384574918152.58890091777536248.614895903705083711.813416: 9.917864022323.21723650512433168.19030338075447383.220955: 7.263053061103.97401982309614307.44133514765453911.856473: 7.954699068324.31586880391256271.16288763096424021.434482: 8.885322305568.7060967688014222.3495265997172433.546475: 9.833102207112.27991168508237172.63625838745884441.154379: 10.2899374251.24375200368108148.67418491324814512.237435: 9.717817866334.0364057157029178.683191175643064611.622339: 10.19941455318.3417606532175153.422321114661324712.981637: 10.8121127353.02528914982014121.28487259370036482.125905: 11.2980060476.0329812280236995.79863310856922492.681942: 7.32428365490.22069071826459304.22964761089133502.083996: 9.67741485474.96364102759367180.80242348809787530.020391: 3.59862346622.30918054635124499.6492209337072541.57375: 9.04937761.94432393798009213.7444748945062553.747128: 9.259248781117.39972650378202202.7362101067971560.376813: 6.48544189731.403560582087437348.22885579658197571.90177: 10.8167152970.31400521276724121.04345627353524582.140262: 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translocation speeds

Duration information not available.

Chimeric read splitting

Dorado performs read splitting when it finds adapter sequences in the middle of the signal. The read is split and the original signal uuid is saved in the 'pi' tag.

No 'pi' tags were found.